酶解用于有效的谷物加工:计算机辅助优化1,3-1,4-β-葡萄糖酶,提高了热稳定性和催化活性
Yi-Fan Zhao1, Ying Zhang1, Ying-Zhi Peng1
1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu 212100, China.
International journal of biological macromolecules
|April 12, 2025
概括
工程β-葡萄糖酶 (M3) 显示了增强的热稳定性和用于谷物加工的活性. 这种改进的酶提供了更好的干物质消化能力,并有助于造过程,显示出重要的工业应用潜力.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 工业微生物学 工业微生物学
背景情况:
- β-葡萄糖酶的有限热稳定性限制了它们在谷物加工中的工业应用.
- 优化酶特性对于高效的工业使用至关重要.
研究的目的:
- 通过计算机辅助设计增强一个1,3-1,4-β-葡萄糖酶 (BisGlu16B_ΔC) 的热稳定性和活性.
- 在模拟的工业应用中评估工程酶的性能.
主要方法:
- 用计算机辅助设计优化能源,以识别热稳定变体.
- 从选定的变种 (T40K,Q53L,S311Y) 中生成一个组合突变 (M3).
- 与野生型 (WT) 相比,M3的热稳定性,特异性活性,催化效率和蛋白酶抵抗性的表征.
主要成果:
- 与WT相比,M3突变体表现出显著改善的热稳定性 (在60°C时t1/2延长126分钟),更高的特异活性 (1.24倍) 和催化效率 (1.18倍).
- 在模拟的胃肠道系统中,M3表现出优异的干物质消化能力 (1.49倍).
- 在酒过程中,M3显著降低了过时间 (1.55倍) 和粘度 (2.37倍),与西兰酶有着强烈的协同效应.
结论:
- 工程化β-葡萄糖酶M3具有增强的热稳定性,活性和工业应用性能.
- 在谷物加工和酒行业中,M3 是一个有前途的候选人,可以有效地应用.
- 在M3的结构修改有助于提高其刚性和催化效率.
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