使用多阶段连续发酵的奇异取性发酵的强化和技术经济评估
Ulises Andrés Salas-Villalobos1, Diego Homma-Dueñas2, Mario A Torres-Acosta2
1Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Eugenio Garza Sada 2501 Sur 64849, Monterrey, Nuevo León, Mexico.
Bioresource technology
|April 12, 2025
概括
多阶段连续反应器 (MSCR) 通过分离生长阶段和提取阶段来增强生产. 这种方法可以提高30%的生产率,并将这种有价值的生物活性化合物的成本降低20%.
科学领域:
- 生物技术是生物技术.
- 微生物生产 微生物生产
- 自然产品 自然产品
背景情况:
- 对天然,安全的生物活性分子,如奇异的需求日益增长.
- 普罗迪吉奥辛表现出抗微生物和抗瘤特性,使其成为一个有趣的化合物.
- 传统的生产方法对于prodigiosin面临的局限性,包括产品抑制和高成本.
研究的目的:
- 为了研究多阶段连续反应器 (MSCR) 的有效性,以提高产量.
- 为提取性发酵优化MSCR参数,包括稀释速率和反应堆体积配置.
- 评估优化MSCR系统的经济可行性,以竞争激烈的生产和生物加工.
主要方法:
- 使用因数设计来评估稀释速率 (0.042 - 0.050小时−1) 和混合反应堆体积配置 (1L - 0.5L).
- 在MSCR系统中采用提取性发酵,以促进产品的连续去除.
- 使用动力方程建模了最佳系统,并在各种稀释速率下评估了经济可行性.
主要成果:
- 在MSCR中将生长阶段和生产/提取阶段分开,显著提高了30%的生产率.
- 与单一连续系统相比,优化的MSCR系统使成本降低了20%.
- 动力建模和经济评估证实了MSCR在生产奇异的可行性.
结论:
- 多阶段连续反应堆 (MSCR) 提供了一个有前途的策略,用于加大生产量.
- 在MSCR系统中的提取性发酵提高了生产率并降低了运营成本.
- MSCR 技术为具有价值的自然产品,如奇迹菌,提供了一条通往更具经济竞争力的生物工艺的途径.
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