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在出生时从胎盘中衍生出人类热囊细胞干细胞

Victoria Karakis¹, John W Britt², Mahe Jabeen¹

¹Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695.

The Journal of biological chemistry

|April 12, 2025

概括

研究人员成功地利用一种新的TUA介质从终产胎盘中获得了人类热囊细胞干细胞 (hTSC). 这些细胞对于胎盘细胞原体 (CTB) 建模至关重要,可以培养和分化,扩大研究可能性.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
发展生物学发展生物学

背景情况:

人类热细胞干细胞 (hTSCs) 对于体外模拟胎盘细胞热细胞 (CTB) 是至关重要的.
以前的衍生方法依赖于第一季度的胎盘或胚胎胚胎胚胎.
标准的多细胞干细胞介质 (TSCM) 含有EGF,CHIR99021,A83-01,SB431542,VPA,以及Y-27632.

研究的目的:

建立一种方法来从产后胎盘CTB中导出hTSCs.
为了研究改性介质 (TUA) 对hTSC衍生和培养的疗效.
评估术语衍生hTSCs的分化潜力和转录组相似性.

主要方法:

从终产胎盘中分离出CTB.
细胞被培养在TUA介质中,一种含有UK5099和富含脂质白蛋白的修饰TSCM.
在随后的培养和差异化研究中,hTSCs被转换为TSCM.

主要成果:

hTSCs成功地从使用TUA介质的术语CTB中衍生出来.
单独使用TSCM或缺乏UK5099或丰富脂质白蛋白无法支持衍生.
术语衍生hTSCs可以在TSCM培养,分化为extravillous和syncytiotrophoblast血统,并显示出与第一季度hTSCs的高转录组相似性.

结论:

这项研究提出了一种新的方法,用于从术后胎盘中导出hTSCs.
这些发现有助于从各种胎盘样本,包括病理样本,轻松地获得hTSCs.
这些术语衍生hTSCs适合用于体外机理学研究在热囊细胞生物学.

关键词:

细胞转移性细胞体.奇异的热囊细胞.人类热囊细胞干细胞胎盘胎盘胎盘胎盘综合性类细胞.

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