尿路食品生物活性物质代谢物的分析和识别的多层次工作流程 第一部分:优化尿液提取
Evangelos Kalampokis1, Theodora Nikou1, Stavros Beteinakis1
1Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece.
Analytica chimica acta
|April 12, 2025
概括
开发改进的尿液制备方法提高了食品生物活性化合物代谢物的识别. 这项研究提供了一个可扩展的工作流程,用于准确的代谢组分析,帮助饮食和健康研究.
科学领域:
- 代谢学 代谢学 代谢学
- 营养科学 营养科学
- 分析化学 分析化学
背景情况:
- 饮食通过食品生物活性化合物 (FBs) 显著影响人类健康和疾病预防.
- 尿液是监测饮食暴露的宝贵生物流体,但由于度低和变异性,代谢物识别具有挑战性.
- 开发可扩展的尿液制备方法对于准确的代谢物分离和结构阐明至关重要.
研究的目的:
- 开发和评估尿液制备方法,以加强食品生物活性化合物代谢物的隔离和识别.
- 评估各种提取和丰富技术对尿液代谢的性能.
- 建立一个工作流程,以确定可靠的代谢物识别和改善人类代谢研究的覆盖范围.
主要方法:
- 来自补充西醇 (HT) 的志愿者的尿液样本使用液液提取 (LLE),固相提取 (SPE) 和树脂丰富 (XAD-4,XAD-7,离子交换) 进行了预处理.
- 用HPLC-DAD/ELSD,UPLC-HRMS和NMR分析了提取物,用于代谢物概况和注释.
- 方法的性能是根据产量,多样性,覆盖范围,选择性,成本,易用性和时间来评估的.
主要成果:
- 在测试方法中,XAD-7树脂和LLE (尿液/EtOAc 1:3) 显示出优异的性能.
- 使用LC-HRMS/MS和NMR实现了内源和外源代谢物的详细识别.
- 协议的性能与涉及的代谢物生物化学途径相关评估.
结论:
- 拟议的工作流适用于代谢物分析和分离,为尿液代谢和FB生物转化提供了重要的见解.
- 这种方法确保了可靠的代谢物识别和高覆盖率,从而更准确地解释了代谢研究.
- 这些发现为研究饮食在人类健康中的作用的分析方法提供了宝贵的投入.
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