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基于激光诱导分解光谱和拉曼光谱的自身免疫性脑炎的血液检测

Zhifang Zhao¹, Wangshu Xu², Geer Teng³

¹School of Optics and Photonics, Beijing Institute of Technology, Beijing, 100081, China; Key Laboratory of Photonic Information Technology, Ministry of Industry and Information Technology, Beijing Institute of Technology, Beijing, 100081, China; National Key Laboratory on Near-surface Detection, Beijing, 10072, China.

Analytica chimica acta

|April 12, 2025

概括

这项研究展示了一种新的方法来诊断自身免疫性脑炎 (AE) 通过融合激光诱导分解光谱 (LIBS) 和拉曼光谱. 这种综合方法可以准确地识别AE血液,为临床诊断提供了一个有前途的工具.

科学领域:

生物医学光谱学生物医学光谱学
医学诊断医学诊断医学诊断
分析化学分析化学

背景情况:

自身免疫性脑炎 (AE) 的发病率正在上升,使临床诊断复杂化.
目前对AE的诊断标准复杂且耗时.
需要快速,准确的AE诊断方法.

研究的目的:

探索使用联合激光诱导分解光谱 (LIBS) 和拉曼光谱来识别血液样本中的自身免疫脑炎 (AE) 的可行性.
从原子和分子的角度分析AE诊断的潜在机制.
开发一种改进的AE诊断方法.

主要方法:

从健康人群和AE患者的血清样本使用LIBS和拉曼光谱分析.
雕刻的网状晶片被用作基板,以最大限度地减少光谱变化.
通过结合LIBS和拉曼数据来创建融合光谱,然后使用相互信息 (MI) 进行特征选择.
长期短期记忆 (LSTM) 模型被用于分类.

主要成果:

总共从119名参与者中收集了1785个LIBS和1785个拉曼光谱.
合并的LIBS-拉曼光谱,包括537个选定的特征,使用LSTM模型在AE诊断中达到95.04%的准确性.
与单个技术相比,这种融合光谱方法显著提高了诊断准确度,并缩短了测试时间.
确定了光谱特征 (如Na,K,) 和细胞因子之间的相关性.

结论:

LIBS和拉曼光谱的融合为识别AE血清提供了一种可行和准确的方法.
这种技术提供了一种快速和最少的侵入性方法,以协助AE的临床诊断.
该研究提供了与AE相关的光谱生物标志物的见解,有助于了解疾病机制.

关键词:

这是AE血清AE血清.细胞因子 (cytokines) 是一种细胞因子.在LIBS中,LIBS是指LIBS.拉曼·拉曼,拉曼·拉曼,拉曼·拉曼.

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