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单核酸多态,基因表达和评估与巴基羊细菌性肺炎相关的免疫,抗氧化和病理参数

Ahmed El Sayed¹, Amani Hafez², Ahmed Ateya³

¹Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo, Egypt. decernes@yahoo.com.

Irish veterinary journal

|April 12, 2025

概括

这项研究确定了绵羊肺炎的关键细菌病原体和毒性因素,揭示了对抗病能力至关重要的免疫和抗氧化基因表达的改变. 这些发现突出了诊断和管理绵羊肺炎的潜在生物标志物.

科学领域:

兽医医学兽医医学兽医医学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

绵羊肺炎在绵羊养殖中带来了重大的经济和健康挑战.
它源于各种传染病原体和环境因素影响绵羊免疫力.

研究的目的:

为了研究肺炎羊的核酸变异,基因表达和血清生物标志物.
用PCR在受影响的绵羊中识别细菌菌株和毒性基因.

主要方法:

对50只健康的母羊和150只肺炎母羊进行了比较分析.
对抽样的细菌学分析和肺组织的组织学检查.
评估基因表达 (SNP,免疫,抗氧化基因) 和血清生物化学参数.

主要成果:

确定的流行细菌:克莱布西埃拉肺炎,帕斯托雷拉多,曼海米亚血清菌, Pseudomonas spp., 菌,链球菌,大肠杆菌.
在关键病原体中检测到毒性基因 (iutA, fimH, toxA).
在受影响的绵羊中,观察到促炎和氧化应激基因 (例如IL-1α,TNF-α,NOS) 的显著上调和抗炎/抗氧化基因 (例如IL-10,SOD1) 的下调.
证实了支气管间歇性和支气管肺炎的组织病理证据.

结论:

肺炎绵羊携带各种具有特定毒性因子的病原体.
免疫和抗氧化剂途径中明显的基因表达模式与肺炎有关.
改变的血清特征和基因调节表明复杂的免疫反应和潜在的疾病生物标志物.

关键词:

抗氧化剂是一种抗氧化剂.巴基羊是巴基羊的一种羊.基因表达基因表达基因表达豁免权是一种豁免权.核酸序列变异的变体肺炎是一种肺炎.

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