相关实验视频

Updated: May 13, 2025

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

简单的结构性,可逆性和化学稳定的pyrene衍生物的pH响应光探针及其应用

Jiawei Tang¹, Muxi Wang², Hongming Li²

¹School of Chemistry, South China Normal University, Guangzhou 510006, PR China; Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou 510006, PR China; Key Laboratory of Theoretical Chemistry of Environment Ministry of Education, South China Normal University, Guangzhou 510631, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|April 13, 2025

概括

新的以烯为基础的探头提供了pH的敏感的比度光检测. 这些探测器在各种pH值范围内工作,并显示出对环境和生物应用的希望.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
生物化学生物化学

背景情况:

二烯衍生物是比度光探针中常见的光体.
现有的探头通常使用单体/异构体状态来识别分析物.
需要新的探测器,具有不同的识别机制.

研究的目的:

开发简单的,单替代的烯衍生物用于比值pH传感.
研究用于pH检测的质子化/脱质子化效应的使用.
评估探测器在环境和生物样本中的性能.

主要方法:

三种烯衍生物的合成:烯-1-ol (Probe1),烯-1-ylboronic 酸 (Probe2) 和烯-1-碳酸 (Probe3).
对pH值变化的比度光反应的表征.
确定pKa值和操作pH范围.
评估光稳定性,可回收利用性和抗离子干扰的性能.

主要成果:

探针1和探针2在性条件下检测pH值 (pH值8.112.1和9.413.1),pKa值分别为8.9和11.1.
探测器3在弱酸性条件下检测pH值 (pH值2.35.2),pKa值为4.8.
所有探测器都显示出出色的光稳定性,可回收利用性和对离子干扰的抗性.
在环境水样和活鱼细胞中成功检测pH值.

结论:

结构简单的烯衍生物可以实现敏感的比度pH检测.
质子化/解质子化机制提供了一个替代单体/异体状态的替代方案.
这些探测器显示了现实世界环境和生物pH监测的潜力.

关键词:

解质子化效应的作用光探测器的光探测器皮勒尼斯金字塔地区的金字塔.电比测量方法电比测量方法它们的pH值为pH.

更多相关视频

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

相关概念视频

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Variables Affecting Phosphorescence and Fluorescence

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...