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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Compounds Essential to Human Function01:25

Compounds Essential to Human Function

5.2K
The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
5.2K
Phosphorylation01:02

Phosphorylation

49.4K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
49.4K
ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

5.1K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.1K
Chemistry of the Cell02:58

Chemistry of the Cell

41.2K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
41.2K
Coupled Reactions01:17

Coupled Reactions

7.4K
Cellular processes such as building and breaking down complex molecules occur through stepwise chemical reactions. Some of these chemical reactions are spontaneous and release energy, whereas others require energy to proceed. Cells often couple the energy-releasing reaction with the energy-requiring one to carry out important cell functions. 
Energy in adenosine triphosphate or ATP molecules is easily accessible to do work. ATP powers the majority of energy-requiring cellular reactions....
7.4K

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相关实验视频

Updated: May 13, 2025

Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

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聚酸盐:一个细胞瑞士军刀.

Akash Rai1, Ursula Jakob2

  • 1Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.

Current opinion in biotechnology
|April 13, 2025
PubMed
概括

无机聚酸盐 (polyP) 是一种多功能生物聚合物,具有从细菌抗压力到哺乳动物疾病等多种功能. 最近的研究揭示了polyP的存在.

科学领域:

  • 生物化学和分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 无机聚酸盐 (polyP) 是一种重要的生物聚合物,其功能作用不断扩大.
  • 它在细菌和哺乳动物中的各种功能,包括抗压力,DNA修复,血液凝结,癌症和神经退行,尚未完全理解.

研究的目的:

  • 审查哺乳动物细胞中聚聚的合成和降解途径.
  • 为了概述polyP的细胞区特异性作用.
  • 要突出最近的发现,如何通过蛋白质相互作用来调解其功能.

主要方法:

  • 关于聚聚合成,降解和细胞作用的文献综述.
  • 对最近研究的分析,重点是聚P-蛋白相互作用.
  • 探索polyP对蛋白质溶解度,脚手架和相分离的影响.

主要成果:

  • 概述了哺乳动物细胞中的PolyP合成和降解途径.
  • 讨论了polyP的细胞区特异性功能.
  • 证据表明,polyP的活动是由其与蛋白质的相互作用介导的,影响其溶解性,脚手架和相分离.

结论:

  • 通过它与蛋白质的相互作用,人们越来越了解PolyP的多样化生物作用.

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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

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相关实验视频

Last Updated: May 13, 2025

Assaying for Inorganic Polyphosphate in Bacteria
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Assaying for Inorganic Polyphosphate in Bacteria

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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

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  • 需要进一步的研究来阐明多聚调节蛋白质行为并控制其功能的精确机制.
  • 了解polyP的蛋白质结合能力可能会为涉及polyP失调的疾病开启新的治疗策略.