来自Grifola frondosa内生植物Burkholderia sp的细胞外多糖的高效生产,结构特征和生物活性
Dongchao Xie1, Ruixue Zhang1, Jiajun Huang1
1National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, China.
International journal of biological macromolecules
|April 13, 2025
概括
来自Grifola frondosa内生细菌的新型外聚糖 (GFB-MP) 显示出对食品病原体具有显著的抗菌特性,并促进益生菌生长. 这种多糖具有在有益健康的食品应用中使用的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 药用中的内性细菌,如Grifola frondosa,代表了新型生物活性化合物的来源.
- 了解这些内植物产生的多糖的组成和功能对于它们的生物技术应用至关重要.
研究的目的:
- 为了识别和分离来自Grifola frondosa的内菌.
- 描述由孤立的Burkholderia sp.产生的一个外聚糖 (GFB-MP). (GFB) 菌株. 菌株. 菌株. 菌株. 菌株. 菌株.
- 评估GFB-MP.的抗菌和益生菌增长促进活动.
主要方法:
- 从Grifola frondosa中分离和识别了内性细菌.
- 发酵优化和产量计算用于外聚糖 (GFB-MP) 生产.
- 分子重量的确定,组成分析 (甲基化,NMR),以及对抗菌和益生菌促进生长活动的评估.
主要成果:
- 这种内性细菌是Burkholderia sp. (GFB) 是从Grifola frondosa.中分离出来的.
- 优化的发酵产生了11.36 g/L的GFB-MP (Mw 432.05 kDa),这是一种以曼诺斯丰富的多糖,主要由银河糖,葡萄糖和曼诺斯组成.
- GFB-MP显著抑制了李斯特菌 (73.4%) 和大肠杆菌 (81.6%),并促进了益生菌生长超过50%.
结论:
- GFB-MP是一种来自Grifola frondosa相关的内菌的外聚糖,具有强大的抗菌和促进生长的特性.
- 其特征结构和生物活性表明GFB-MP作为有益健康食品中的功能成分的潜力.
- 进一步研究来自G. frondosa的内菌衍生的多糖类可以阐明共生相互作用和生理作用.
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