相关实验视频

Updated: May 13, 2025

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

通过METTL3介导的N6-甲基氨酸修饰有助于血管化

Long Li¹, Quanyou Chai¹, Chunling Guo²

¹Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China; Zhejiang Key Laboratory of Cardiovascular Intervention and Precision Medicine, Hangzhou, China; Engineering Research Center for Cardiovascular Innovative Devices of Zhejiang Province, Hangzhou, China; Department of Cardiology, The Second Hospital of Shanxi Medical University, Taiyuan, China.

Journal of molecular and cellular cardiology

|April 13, 2025

概括

在慢性病 (CKD) 中,N6-adenosine-methyltransferase-like 3 (METTL3) 通过增加m6A修饰来促进血管化 (VC). 奎尔丁抑制METTL3,减轻了VC.

科学领域:

生物化学生物化学
分子生物学分子生物学
心血管研究研究心血管研究

背景情况:

血管化 (VC) 是慢性病 (CKD) 中的一个显著的心血管并发症.
在VC中N6-甲基氨酸 (m6A) 修饰的作用和机制在很大程度上仍未被探索.
类似N6-adenosine-methyltransferase-3 (METTL3) 是一个关键的m6A编写酶.

研究的目的:

阐明METTL3在血管化 (VC) 中的作用.
调查METTL3在VC病变发生过程中的潜在分子机制.
确定针对VC的METTL3的潜在治疗策略.

主要方法:

在化血管光滑肌细胞 (VSMCs) 中对METTL3表达的生物信息分析.
在体外 (VSMCs) 和体内 (CKD小鼠模型) 实验验证METTL3在VC中的作用.
对m6A水平,沉积和信号通路的评估 (PTEN/AKT).
RNA免疫沉降 (MeRIP) 试验以确认PTEN mRNA的m6A修饰.
分子对接和DARTS测试以确定奎尔素作为METTL3抑制剂.

主要成果:

在化VSMC和CKD小鼠的VC模型中,METTL3表达被上调.
过度表达METTL3增强了m6A水平并促进了化,而敲击抑制了这些效应.
通过 PTEN/AKT 途径通过诱导 PTEN mRNA 通过 m6A 修改降解,METTL3 促进了 VC.
奎尔赛丁被确定为METTL3的天然抑制剂,在体外和体内有效减轻VC.

结论:

通过m6A修饰,METTL3在血管化 (VC) 的病原发生中发挥着关键作用.
通过METTL3介导的PTENmRNA的m6A修饰有助于VC的发展.
在慢性病 (CKD) 患者中,METTL3代表了管理VC的有希望的治疗标.
奎尔丁通过抑制METTL3活性,显示出作为VC治疗剂的潜力.

关键词:

抑制剂的抑制剂在METTL3中,METTL3是METTL3的第一个类型.奎尔丁 (Quercetin) 是一种青素.血管化是因为血管化.m6A 修改的修改

更多相关视频

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

Analysis of Extracellular Vesicle-Mediated Vascular Calcification Using In Vitro and In Vivo Models

Published on: January 27, 2023

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...