相关实验视频

Updated: May 13, 2025

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

基因组对Klebsiella pneumoniae ST11中核心毒性因子的比较基因组分析,基于全球基因组数据库

Ruyu Yan¹, Han Shen¹, Kai Zhou²

¹Department of Laboratory Medicine, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.

Microbial pathogenesis

|April 13, 2025

概括

耐卡巴胺的Klebsiella pneumoniae (CRKP) ST11菌株显示了丰富的yersiniabactin (Ybt) 基因群,增强了毒性和适应性. 主导的K64和K47血清型携带更多的Ybt和卡巴酶基因,包括blaKPC-2.

科学领域:

微生物学与传染病的研究
基因组学和分子流行病学
公共卫生和流行病学

背景情况:

耐卡巴尼姆的克莱布西拉肺炎 (CRKP) ST11的全球传播是严重的公共卫生问题,因为其具有很高的传染性,多药性耐药性和毒性.
对导致CRKP ST11病原性和适应性的核心毒性因子 (VF) 的有限理解,阻碍了有效的控制策略.

研究的目的:

为了描述全球K. pneumoniae ST11菌株的毒性概况.
研究K. pneumoniae ST11中主要毒性因子,血清型和碳烯酶编码基因之间的关联.

主要方法:

来自江苏的62个临床K. pneumoniae分离物的基因组测序,补充了来自GenBank的全球菌株.
多位置序列类型 (MLST) 识别序列类型 (ST) 和比较基因组分析以确定关键毒性因素.
遗传学分析以探索遗传关系,并分析血清型和碳烯酶基因分布.

主要成果:

在临床分离 (46/62) 和全球 (1571/11,429) 中,ST11是最常见的克隆,其次是ST258,ST15和ST512.
耶尔西尼亚巴克丁 (Ybt) 基因群,包括ybtT,ybtX,fyuA,ybtP,ybtU,ybtS,ybtA,ybtQ,ybtE, irp1和 irp2,在ST11菌株中显著丰富,特别是K64和K47血清型的菌株.
主要的碳烯酶基因是blaKPC-2 (30.8%),blaNDM-1 (5.3%) 和blaOXA-48 (2.9%),而K64 (38.0%),K47 (19.5%) 和K24 (1.8%) 血清型是最常见的.

结论:

在ST11株中Ybt集群和fyuA的丰富可能有助于增强殖民和免疫逃避.
主导的K64和K47血清型与Ybt和carbapenemase基因,特别是blaKPC-2的更高患病率有关.
调查结果揭示了CRKP-ST11的特定区域传播模式,强调需要加强监测和有针对性的控制措施.

关键词:

进行比较的基因组学.克莱布西拉肺炎是一种肺炎.人类遗传学树 (Phylogenetic Tree) 是一个遗传学树.ST11 ST11 在这里.血清型类型血清型类型病毒性基因是病毒性基因.

更多相关视频

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Published on: January 7, 2019

相关概念视频

Genomic DNA in Prokaryotes

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...

Antibiotic Selection

Antibiotic Selection

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Genomic DNA in Eukaryotes

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.