评估超临界CO2治疗诱导的β-乳糖球蛋白的二次结构变化
Rahul Venkatram1, Silvia de Lamo Castellvi2, Luis Rodriguez-Saona1
1Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210.
Journal of dairy science
|April 13, 2025
概括
超临界CO2处理改变了全脂奶粉中的β-乳糖球蛋白 (β-LG) 结构,降低了抗原性. 这种处理通过化和糖蛋白形成独特地改变了蛋白质,影响了其二次结构.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物物理化学 生物物理化学
背景情况:
- β-乳糖球蛋白 (β-LG) 是牛奶中主要的一种乳清蛋白.
- 了解蛋白质结构变化对于食品加工和过敏性至关重要.
- 超临界CO2 (ScCO2) 是一种新兴的食品改造技术.
研究的目的:
- 研究超临界CO2 (ScCO2) 对全奶粉 (WMP) 中β-LG的二次结构的影响.
- 为了将结构变化与降低的抗原性相关联.
- 为了识别由ScCO2处理引起的独特的翻译后修改.
主要方法:
- 全奶粉 (WMP) 在63°C/200 bar和75°C/300 bar处经过超临界CO2 (ScCO2) 处理.
- 通过同电沉和膜过进行β-LG隔离.
- 使用紫外线吸收,循环二极化 (CD) 和富里埃变换红外光谱法 (FTIR) 的二次结构分析.
- 用三明治ELISA测量的抗原性降低.
- 使用Orbitrap聚变液体色谱-MS/MS.识别的翻译后修改.
主要成果:
- ScCO2治疗显著降低了β-LG抗原性 (高达54.75%).
- 结构分析显示β-LG的展开,增加了表面的疏水性和Trp残留的暴露.
- 磁盘光谱显示α螺旋和随机线圈增加,β片减少.
- 轨道合LC-MS/MS检测到酸和乳糖,表明SCO2处理的独特的翻译后修改 (化和糖化).
- 糖蛋白形成和化显著影响了FTIR胺I和II频段.
结论:
- 超临界CO2 (ScCO2) 处理会导致β-LG二次结构的显著形状变化.
- 这些结构变化与蛋白质抗原性降低有关.
- 化和糖蛋白形成是新的,ScCO2特定的翻译后修改,影响β-LG结构和功能.
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