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在人类胚胎囊中分析线粒体DNA量化和辅助生殖结果的分析

Jing Zhang¹, Fang Li¹, Dan Kuai¹

¹Reproductive Medical Center, Department of Gynecology and Obstetrics, General Hospital of Tianjin Medical University Tianjin 300052, China; Tianjin Key Laboratory of Female Reproductive Health and Eugenics, Tianjin Medical University General Hospital, Tianjin, China.

Reproductive biomedicine online

|April 13, 2025

概括

在皮活检中,线粒体DNA (mtDNA) 拷贝数在euploid胚胎中低于aneuploid胚胎. 然而,mtDNA水平并不能预测辅助生殖技术 (ART) 中的怀孕结果.

科学领域:

生殖生物学生殖生物学
遗传学是一个遗传学.
胚胎学胚胎学

背景情况:

人类肌皮细胞中的线粒体DNA (mtDNA) 含量可能会影响胚胎发育和辅助生殖技术 (ART) 的结果.
研究mtDNA水平与关键胚胎变量之间的相关性对于理解ART成功至关重要.

研究的目的:

为了确定体皮活检中的线粒体DNA (mtDNA) 含量是否与胚胎特征和辅助生殖技术 (ART) 结果相关.
评估mtDNA拷贝数对IVF成功的预测价值.

主要方法:

分析了来自136个植入前遗传测试 (PGT) 周期的462个皮皮组织活检.
使用全基因组放大和下一代测序来量化mtDNA水平.
相关性分析与胚胎繁体化,胚芽细胞形态和ART结果.

主要成果:

胚胎平分化与线粒体DNA复制数 (MCN) 有意义地相关 (P < 0.0001).
无胚胎胚胎的mtDNA数量比euploid胚胎的mtDNA数量更高,不论胚胎细胞的形态如何.
在MCN中没有发现关于怀孕结果的统计学上显著差异 (P = 0.619).

结论:

欧体胚胎与无体胚胎相比,其mtDNA复制数量明显较低.
皮活检中的mtDNA拷贝数可能不是试管婴儿中成功临床结果的可靠预测指标.
需要进一步的研究来探索mtDNA在胚胎能力中的作用.

关键词:

艺术艺术艺术艺术胚胎形态学胚胎形态学欧普洛伊迪症是什么意思?结果的结果.托菲克托德的细胞细胞.我们的mtDNA mtDNA

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