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相关概念视频

CRISPR01:59

CRISPR

48.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.3K

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相关实验视频

Updated: May 13, 2025

Synthetic Spider Silk Production on a Laboratory Scale
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蜘蛛眼的开发编辑和丝纤维工程使用CRISPR-CasPR.

Edgardo Santiago-Rivera1, Thomas Scheibel1,2,3,4,5

  • 1Department of Biomaterials, University of Bayreuth, Prof.-Rüdiger-Borman Strasse 1, 95448, Bayreuth, Germany.

Angewandte Chemie (International ed. in English)
|April 14, 2025
PubMed
概括

克里斯普尔基因编辑首次成功应用于蜘蛛,在后代中产生基因淘汰和淘汰突变. 这项研究使蜘蛛的遗传修饰能够用于发育遗传学和材料科学应用.

关键词:
眼睛的损失 眼睛的损失光丝纤维是一种光丝纤维.基因型 基因型 基因型敲门入门的服务.淘汰赛 - 淘汰赛 - 淘汰赛主要的安普拉特丝丝

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Microdissection of Black Widow Spider Silk-producing Glands
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Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
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相关实验视频

Last Updated: May 13, 2025

Synthetic Spider Silk Production on a Laboratory Scale
13:36

Synthetic Spider Silk Production on a Laboratory Scale

Published on: July 18, 2012

26.6K
Microdissection of Black Widow Spider Silk-producing Glands
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Microdissection of Black Widow Spider Silk-producing Glands

Published on: January 11, 2011

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Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
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科学领域:

  • 遗传学 遗传学 是一个
  • 发展生物学 发展生物学
  • 材料科学 是一种材料科学.

背景情况:

  • CRISPR-Cas9是一种精确的基因组编辑工具,具有广泛的应用.
  • 以CRISPR为基础的基因编辑以前没有在蜘蛛中报告过.

研究的目的:

  • 为了证明CRISPR-Cas9基因编辑在蜘蛛中的可行性.
  • 为了研究基因功能,并使蜘蛛中的丝蛋白功能化.

主要方法:

  • 在父母蜘蛛中进行了CRISPR介导的微注射.
  • 在后代中诱导了敲除 (KO) 和敲进 (KI) 突变.
  • 眼基因是KO的目标,光蛋白是KI的目标.

主要成果:

  • 眼基因的KO导致后代完全失去眼睛,证实了它在眼睛发育中的作用.
  • 将单体红色光蛋白 (mRFP-KI) 的 KI 转化为丝基因,产生红色光丝纤.
  • 丝组装不受KI突变的影响,证明了功能化的可行性.

结论:

  • 克里斯普尔-Cas9基因编辑现在适用于蜘蛛.
  • 这种技术可以研究发育遗传学和创造新的丝材料.