OPTIMATRIX v2.0:优化的协议,以减轻微生物的花在微矩阵生物反应器平台,用作活体人类远端结肠模型
Arghya Mukherjee1,2, Nicola Ferremi Leali3, Elisa Salvetti4
1Teagasc Food Research Centre, Moorepark, Fermoy, Cork, Ireland.
MethodsX
|April 14, 2025
概括
优化微矩阵生物反应器用于ex vivo结肠模型涉及使用MacFarlane.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 生物技术是生物技术.
背景情况:
- 微矩阵生物反应器是人类远端结肠的实体模型.
- 在这个模型中,大肠杆菌的繁荣和微生物多样性的丧失是挑战.
研究的目的:
- 确定微矩阵生物反应器的最佳条件,以减轻大肠杆菌的繁荣,并保护肠道微生物的多样性.
- 为改善ex vivo结肠模型实验提供关键见解.
主要方法:
- 测试四种便发酵介质组成.
- 比较组合与个人捐赠者便样本.
- 评估长时间的实验持续时间 (长达96小时).
主要成果:
- 麦克法莱恩和其他人. 介质的组成最好地缓解了大肠杆菌的繁荣,并保持了微生物多样性.
- 与单个样本相比,聚合的便样本增强了肠道微生物群的多样性.
- 长时间的实验 (长达96小时) 可能有利于苛刻的肠道微生物.
结论:
- 麦克法莱恩等人. 介质,聚合便样本和延长实验持续时间是优化ex vivo结肠模型的建议.
- 这些优化对于准确研究肠道微生物群的动态和 in vitro 功能至关重要.
相关概念视频
01:30Microbial Wastewater Treatment
Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:30Designing Growth Media for Bioreactors
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...