毛细管振动尖端喷雾电离的优化,用于三重DNA原生质谱的原生质谱
Sultan Mahmud1, Vikum K Dewasurendra2, Chandrima Banerjee1
1Department of Chemistry, West Virginia University, Morgantown, West Virginia 26506, United States.
ACS omega
|April 14, 2025
概括
毛细管振动尖端喷雾电离 (cVSSI) 优化了DNA三倍离子生产,用于本地质谱 (MS). 中等应用电压和受控的入口温度增强了所需的三倍离子,最大限度地减少了敏感的寡核酸分析的 adducts.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 原生质谱法 (MS) 能够在没有变质条件的情况下研究生物分子结构.
- 离子生产效率对于敏感检测大型寡核酸,如DNA三重复的至关重要.
- 毛细管振动尖端喷雾电离 (cVSSI) 是一种用于MS电离的新技术.
研究的目的:
- 通过使用cVSSI,研究应用电压和加热的输入传输管温度对DNA三倍离子产生的影响.
- 优化cVSSI参数,以增强本地MS分析负离子模式中的大型寡核酸.
主要方法:
- 使用毛细管振动尖端喷雾电离 (cVSSI) 来将样品引入质谱仪.
- 系统地改变应用电压 (-900 到 -1500 V) 和加热的输入传输管温度 (250 到 450 °C).
- 使用质谱分析分析了DNA三倍离子 (Tri),三倍 adduct 离子 (Tri+ad) 和DNA三倍碎片离子 (Tri-fr) 的丰度.
主要成果:
- 与较高的电压相比,中等应用电压 (-900 至 -1000 V) 显著增加了所需的三倍离子强度 (高达 ~ 260 倍).
- 在300-400°C之间的最佳毛细管入口温度最大化了三倍离子丰度,对于不同的电荷状态具有特定的最佳值.
- 较高的温度 (450°C) 导致离子丰度下降,而碎片离子因源内激活而达到400°C的峰值.
结论:
- 对于cVSSI的最佳条件是使用中等电压 (~-900V) 和300-350°C的入口温度.
- 这些优化的参数增强了原生DNA三倍离子的产生,这对于敏感分析至关重要.
- 这些发现为使用cVSSI在负离子模式下使用大型寡核酸的敏感本源MS奠定了基础.
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