根据广泛向的代谢学,网络药理学,分子对接和细胞实验,对不同种植环境下种植的Dendrobium officinale茎的质量差异进行多维评估
Yingyue Hou1, Guangying Du1, Jing Li1
1School of Information Engineering, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Frontiers in plant science
|April 14, 2025
概括
种植环境显著改变Dendrobium officinale干代谢物,影响治疗效果. 石生长环境产生优质的茎,具有独特的代谢物概况,为质量评估提供了一种新的方法.
科学领域:
- 植物化学和代谢学
- 传统中国医药 传统中国医药
- 药理学 药理学是指药理学的学科.
背景情况:
- 临灭绝的药用植物Dendrobium officinale表现出基于种植的质量差异.
- 了解这些质量差异的生物化学基础对于其药物和食品应用至关重要.
- 目前对培养依赖的代谢物变异及其对治疗疗效的影响的知识有限.
研究的目的:
- 调查不同种植环境 (石,树,温室) 对Dendrobium officinale茎的代谢物概况的影响.
- 通过网络药理学和分子对接,识别关键的差异代谢物及其潜在的治疗点.
- 探索代谢物组成,种植环境和Dendrobium officinale的治疗作用之间的关系.
主要方法:
- 广泛向的代谢学 (UPLC-MS/MS) 用于识别和量化来自不同环境的树干中的代谢物.
- 网络药理学分析差异化代谢物的核心标.
- 分子对接以验证代谢物-标相互作用并识别质量标记.
- 在体外实验中评估对慢性缩性胃炎细胞的治疗效果.
主要成果:
- 确定了1929种初级和二级代谢物;58种在石生长环境中得到了上调.
- 上调的代谢物包括7种氨基酸和18种黄,尼可提佛林和异基显示强烈的结合目标MAOA和TNF.
- 石头形茎对慢性缩性胃炎细胞表现出优越的保护作用,与改变的代谢物-点相互作用有关.
结论:
- 种植环境显著影响Dendrobium officinale茎的代谢物组成和含量.
- 代谢物概况及其目标相互作用的变化与治疗疗效的差异相关.
- 这项研究为评估Dendrobium officinale质量和理解环境驱动的治疗变异提供了一个新的框架.
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