在第二个近红外窗口进行双复合体内生物分子成像,以检测免疫治疗反应
Yupeng Sun1,2, Rui Li1,3, Yike Cai1,3
1The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, P. R. China.
Theranostics
|April 14, 2025
概括
这项研究引入了一种新的双复合NIR-II分子成像技术,用于同时非侵入性监测CD8+T细胞和瘤中的PD1水平. 这种方法有助于瘤免疫类型和预测抗瘤治疗反应.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 免疫学 免疫学 免疫学
- 纳米技术纳米技术
背景情况:
- 瘤透的CD8+ T细胞和编程死亡-1 (PD1) 对于瘤免疫类型和治疗决策至关重要.
- 在第二个近红外窗口 (NIR-II) 中进行非侵入性光学成像,可以在体内深入组织,以研究生物过程.
- 目前的NIR-II成像仅限于单探头应用,阻碍了对多个生物标志物的同时分析.
研究的目的:
- 开发一种双复合NIR-II分子成像方法,用于同时,非侵入性地监测CD8+T细胞和瘤中的PD1水平.
- 为了使瘤免疫类型的动态评估,并指导治疗策略.
- 克服单探头NIR-II成像用于多重生物标志物分析的局限性.
主要方法:
- 在NIR-IIa窗口中使用氨酸绿 (ICG) 和在NIR-IIb窗口中使用PbS/CdS量子点 (QD) 开发了一种双复合NIR-II成像方法.
- 使用PD1受体标记ICG (ICG-Apt-PD1) 和CD8受体标记QD (QDs@Apt-CD8) 进行同时准和成像.
- 集成的非重叠的光辐射用于多重检测CD8+T细胞和PD1水平在体内.
主要成果:
- QDs@Apt-CD8显示出高选择性和特异性,用于瘤透CD8+T细胞的体内成像.
- 双复合成像能够动态监测瘤内的PD1水平和CD8+T细胞计数.
- 在瘤类型中观察到PD1和CD8+T细胞的异质分布,显示出不同的免疫类型.
- 发现低PD1和高CD8+T细胞水平与更好的抗瘤效果相关.
结论:
- 在体内开发的非侵入性NIR-II分子成像技术补充了ex vivo活检方法.
- 这种技术可以为体内瘤免疫评分算法做出贡献,以精确预测免疫治疗反应.
- 能够更全面地了解瘤微环境和癌症治疗患者分层.
相关概念视频
Double Resonance Techniques: Overview
154
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Spin decoupling is usually achieved by...
154
¹H NMR: Complex Splitting
1.2K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.2K
Tandem Mass Spectrometry
787
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
787
Phase II Conjugation Reactions: Overview
116
Conjugation, a key component of phase II biotransformation reactions, is a vital process in drug detoxification. It involves transferring endogenous substances like glucuronic acid, sulfate, and glycine to drugs or their metabolites formed in phase I reactions. These conjugation reactions, often catalyzed by specific enzymes, transform potentially harmful metabolites into inactive, water-soluble forms easily excreted in urine or bile. By enhancing polarity and eliminating pharmacological...
116
¹H NMR: Pople Notation
1.6K
The Pople nomenclature system classifies spin systems based on the difference between their chemical shifts. Coupled spins are denoted by capital letters with subscripts indicating the number of equivalent nuclei. When the coupled nuclei have well-separated chemical shifts, they are assigned letters that are far apart in the alphabet, such as A and X. When the difference in chemical shifts is small, coupled nuclei are named using adjacent letters of the alphabet (AB, MN, or XY).
A proton...
A proton...
1.6K


