丸丰富的Socs7对小鼠的精子生成和雄性生育不至关重要
Nianchao Zhou1, Junpeng Deng2, Hui Zhou1
1Human Reproductive and Genetic Center, Affiliated Hospital of Jiangnan University Wuxi 214062, Jiangsu, China.
American journal of translational research
|April 14, 2025
概括
细胞因子信号传递7抑制剂 (SOCS7) 在丸中高度表达,但其缺乏不会影响小鼠的雄性生育能力或精子生产. 这项研究澄清了SOCS7的原因.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 哺乳动物遗传学 哺乳动物遗传学
背景情况:
- 细胞因子信号传递7抑制剂 (SOCS7) 是细胞过程中的关键调节剂,如胰岛素抵抗和炎症.
- 在男性生殖细胞和精子生成中SOCS7的特定功能还不清楚.
研究的目的:
- 研究SOCS7在小鼠精子生成中的作用.
- 探索男性生殖细胞中SOCS7的潜在调节机制.
主要方法:
- 对丸中Socs7表达的生物信息学分析.
- 通过使用CRISPR/Cas9.9生成Socs7敲杀小鼠.
- 丸形态的组织学和免疫组织化学评估.
- 通过计算机辅助精子分析 (CASA) 评估精液质量.
- 使用 TUNEL 试验评估丸亡.
主要成果:
- Socs7在人类和小鼠丸中显示出高表达.
- 与野生类型相比,Socs7淘汰赛小鼠的生育能力正常,丸形态或精子质量没有显著变化.
- 在Socs7淘汰赛小鼠中没有观察到丸亡的显著变化.
结论:
- 尽管丸表达很高,但SOCS7对于维持雄性生育或小鼠精子生成并非必不可少.
- 这些发现有助于了解SOCS7在男性生殖中的作用,并防止冗余研究.
相关概念视频
01:27Testes: Histology
491
A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
The spermatogenic cells, responsible for producing sperm, are...
491
01:41Spermatogenesis
101.8K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
101.8K