体特异运输 (pst) 位点的邻近的ATP结合蛋白编码基因具有非重叠的细胞功能
Christopher M Healy1,2, Evelyn A Pham1,2, Keane J Dye1,2
1Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Journal of bacteriology
|April 14, 2025
概括
在Enterococcus faecalis中,有两种用于无机酸盐 (Pi) 进口的PstB ATPase. 对于Pi吸收来说,PstB2是必不可少的,而PstB1则起着辅助作用,突出显示了类似基因的功能分歧.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 无机 (Pi) 对微生物生命至关重要.
- Pst-PhoU系统促进了细菌中高亲和度,低速度的Pi进口.
- PstB ATPase为这种Pi进口机制提供动力.
研究的目的:
- 调查Enterococcus faecalis.中的两个相邻的PstB ATPase对应物 (PstB1和PstB2) 在Enterococcus faecalis.中的不同作用.
- 为了确定Firmicutes中的tandem pstB基因的功能意义.
主要方法:
- 对E. faecalis pstB1和pstB2删除突变的比较遗传分析.
- 在低Pi条件下评估生长缺陷.
- 测量无机酸盐吸收和性酸酶活性.
- 通过基因过度表达进行补充研究.
主要成果:
- 删除pstB2严重抑制了低Pi介质的生长,而删除pstB1导致了较轻微的生长缺陷.
- 对于无机酸盐的进口,PstB2是必不可少的,这一点在突变物体中减少了Pi摄入量和增加了性酸酶活性.
- 过度表达PstB2恢复了pstB1突变体中的Pi进口,但PstB1无法拯救一个pstB2突变体.
结论:
- E. faecalis pstB2编码了无机酸盐进口所必需的初级ATPase.
- 在Pi进口中,PstB1 ATPase具有次要的,非必不可少的作用,可以通过PstB2.2进行补偿.
- 这项研究揭示了E. faecalis.中PstB同类物之间的功能专业化.
相关概念视频
Allosteric Proteins-ATCase
5.6K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.6K
The ADP/ATP Carrier Protein
3.1K
ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
3.1K
ATP Synthase: Mechanism
13.8K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
13.8K
Exon Recombination
3.5K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.5K
Cell Specific Gene Expression
13.4K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.4K
ATP Synthase: Structure
11.8K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
11.8K


