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相关概念视频

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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相关实验视频

Updated: May 13, 2025

Manipulation of Gene Function in Mexican Cavefish
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序列编码的Morpholinos的构想和合成

Benoit Pousse1,2, Abdelaziz Al Ouahabi2, Paul N W Baxter1,2

  • 1CNRS, ISIS, Université de Strasbourg, 8 allée Gaspard Monge, Strasbourg, 67000, France.

Chemistry (Weinheim an der Bergstrasse, Germany)
|April 14, 2025
PubMed
概括

固态形态分子化学可以合成序列定义的寡合物. 这种方法允许可靠地解码和检索存储在这些新型生物聚合物中的二进制信息.

关键词:
数字聚合物数字聚合物化学是的化学.测序控制的聚合物.测序的测序是指测序的测序.固相合成的固相合成方法

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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
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科学领域:

  • 合成有机化学 合成有机化学
  • 聚合物化学 聚合物化学
  • 化学生物学是化学生物学.

背景情况:

  • 对新型应用来说,对序列定义的非生物学寡合体的开发至关重要.
  • 固相合成为控制的寡合体构造提供了一个平台.

研究的目的:

  • 探索固体相形态分子化学,用于合成序列定义的非生物学寡合物.
  • 建立一个二进制系统,在寡合体内编码信息.
  • 为了验证存储的二进制消息的解码和检索.

主要方法:

  • 合成和表征两种具有不同的编码替代体的morpholino comonomers (0和1).
  • 使用连接和去保护步骤的重复循环来合成寡合物的固体相.
  • 通过结合一个限制步骤来确保统一性来优化合成.
  • 使用液态染色体质谱法 (LC-MS) 进行合成的寡合物的表征.
  • 使用双重质谱法 (MS/MS) 分析寡合体碎片化模式.

主要成果:

  • 通过使用二进制的Morpholino字母表成功合成了序列定义的寡合体.
  • 证明了结合一个限制步骤可以产生统一的编码序列.
  • LC-MS和MS/MS分析证实了合成的寡合物的结构和序列.
  • 实现了完整的序列覆盖和存储的二进制消息的可靠解码.

结论:

  • 固体阶段的Morpholino化学提供了一个强大的方法,用于创建序列定义的非生物学寡合体.
  • 开发的二进制系统有效地编码并允许检索数字信息.
  • 这种方法对需要特定序列的分子信息存储的应用具有前景.