在TD-GC×GC/ToFMS分析之前,对体味采样采样采样阶段的研究
Elsa Boudard1, Lisa Fisson2, Nabil Moumane3
1UMR CBI, Laboratoire des Sciences Analytiques, Bioanalytiques et Miniaturisation, ESPCI Paris, PSL Research University, 10 rue Vauquelin, 75231, Paris, Cedex 05, France. elsa.boudard@espci.fr.
Analytical and bioanalytical chemistry
|April 14, 2025
概括
标准化体臭分析需要有效的采样. 被动采样枕 (PSP) 和PowerSorb®对挥发性有机化合物 (VOC) 具有很高的回收和可重复性,这对于医学诊断至关重要.
科学领域:
- 分析化学 分析化学
- 生物医学科学 生物医学科学
- 环境科学 环境科学
背景情况:
- 身体气味分析涉及具有诊断潜力的复杂挥发性有机化合物 (VOC).
- 缺乏标准化的方法,阻碍了体臭分析的进展.
- 由于采样技术的多样性,如气色谱-质谱 (GC-MS) 等当前的方法产生了可变的结果.
研究的目的:
- 为了比较五个直接体臭采样阶段的分析洁净度和VOC捕获/释放效率.
- 为了确定最佳的采样方法可靠的体臭分析.
- 用真实体臭样本验证发现,并确定最佳采样持续时间.
主要方法:
- 五个采样阶段的比较:布,玻璃珠,PowerSorb®,Getxent®微管和被动采样枕头 (PSP).
- 用于简化协议和尽量减少污染/样本损失的热解吸.
- 使用全面的二维气体染色学加上飞行时间质谱学 (GC×GC/ToFMS) 的高灵敏度分析.
主要成果:
- 被动采样枕 (PSP) 实现了95%的平均回收率,变化率为22%.
- PowerSorb®的平均恢复产率为71% ,变化率为10%.
- 对真实体臭样本的最佳采样持续时间估计在30分钟至1小时之间.
结论:
- PSP和PowerSorb®是用于体臭分析的有效采样解决方案.
- 缺乏分析清洁性的吸附剂相,如布,对准确的VOC检测构成风险.
- 标准化采样方法对于在医学和诊断中推进体臭分析至关重要.
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