对关键基因和与口腔状细胞癌Cal27细胞中西斯普拉丁耐药性相关的关键基因和途径的转录组分析

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Updated: May 13, 2025

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

Yu Wang^1,2, Qiwei Zhao^1,2, Long Ding^1,2

¹Department of Basic Medicine, Hubei University of Chinese Medicine, Hongshan District, 16 Huangjiahu West Road, Wuhan, 430065, Hubei, China.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

|April 14, 2025

概括

这项研究确定了瘤亡因子 (TNF),转化生长因子β1 (TGFB1) 和表皮生长因子 (EGF) 作为驱动口腔状细胞癌 (OSCC) 中西斯普拉丁耐药性的关键基因. 针对这些基因可能会改善OSCC患者的治疗结果.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

口腔状细胞癌 (OSCC) 呈现出不良的恢复和化学抵抗.
在OSCC耐药性背后的分子机制仍然不完全理解.

研究的目的:

阐明OSCC中西斯普拉丁耐药性的分子机制.
确定涉及化学抵抗的关键基因和途径,以潜在的治疗向.

主要方法:

建立了一种抗西斯普拉丁的OSCC细胞系 (Cal27R).
使用了转录组分析,生物信息学 (GO,KEGG,PPI),TCGA数据库查询和分子生物学技术.
在人类舌头癌组织和通过基因淘汰实验中得到验证的结果.

主要成果:

鉴定了1927个差异表达基因 (DEG),主要在TNF和MAPK信号通路中.
提升的TNF,TGFB1和IL1B表达与药物耐药性相关;EGF和FOS的下调.
证实TNF,TGFB1和EGF是西斯普拉丁耐药性的关键驱动因素,其调节会影响药物敏感性和患者预后.

结论:

在OSCC中,TNF,TGFB1和EGF是关键基因,与西斯普拉丁耐药性和不良预后有关.
TNF和MAPK信号通路在调解化学抵抗中起着关键作用.
这些发现为开发针对OSCC耐药性的新型诊断和治疗策略提供了基础.

关键词:

西斯普拉丁 (Cisplatin) 是一种药物耐药性药物耐药性药物耐药性口腔状细胞癌的癌症.在RNA-Seqq.

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