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Updated: May 13, 2025

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通过抗生素载多隔间菌体的表面固定来增强抗菌保护

Shabnam Tarvirdipour^1,2, S Narjes Abdollahi¹, Joachim Köser³

¹Department of Chemistry, University of Basel, Mattenstrasse 22, Basel-4058, Switzerland. cornelia.palivan@unibas.ch.

Journal of materials chemistry. B

|April 14, 2025

概括

研究人员为医疗器械涂层开发了含有里芬素的多隔间微粒 (RIF-MCMs). 这些RIF-MCM通过释放抗生素和增加表面粗度来减少细菌感染,显示出预防植入物相关感染的希望.

科学领域:

生物材料科学生物材料科学
传染性疾病传染性疾病
纳米技术纳米技术

背景情况:

抗生素耐药性细菌感染是一个日益增长的全球健康威胁,通常与医疗设备上的生物膜有关.
现有的治疗方法很难有效地对抗局部感染和植入物表面上的生物膜形成.

研究的目的:

开发和评估用于局部抗微生物治疗的表面固定的里芬素载荷多分区菌体 (RIF-MCMs).
调查双重功能抗微生物战略,结合持续的抗生素释放和被动的抗生物膜机制.

主要方法:

优化RIF-MCMs的物理化学特性.
使用石英晶体微平衡和原子力显微镜,将RIF-MCM固定在玻璃基板上.
在生理温度 (37°C和42°C) 下持续释放抗生素的评估.
评估表面粗度及其对细菌粘附和生物膜形成的影响.
在体外对黄金葡萄球菌进行抗微生物测试和对哺乳动物细胞进行细胞毒性评估.

主要成果:

在表面固定的RIF-MCM中,Rifampicin持续释放.
经过RIF-MCM修改的表面显示,金黄色葡萄球菌的生存能力下降了98%,殖民地形成减少了三级.
增强的表面粗度被动地阻碍了细菌的粘附和生物膜的形成.
RIF-MCMs对哺乳动物细胞的细胞毒性最小.

结论:

固定在表面的RIF-MCM为局部治疗应用提供了一种双重作用的抗菌方法.
这一策略有效地降低了细菌的生存能力和殖民地形成,同时防止了生物膜的发展.
开发的RIF-MCM涂层有望预防医疗器械相关感染和生物膜形成,具有低哺乳动物细胞毒性.

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