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对Hansenula polymorpha产生的52型人类乳头瘤病毒病毒样颗粒的表达,净化和免疫性研究

Sheila Chairunnisa¹, Apon Zaenal Mustopa², Budiman Bela³

¹Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia; Master's Programme in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta, 10430, Indonesia.

Biologicals : journal of the International Association of Biological Standardization

|April 14, 2025

概括

一种使用酵母产生的L1病毒样颗粒 (VLPs) 的新型人类乳头瘤病毒52型 (HPV 52) 候选疫苗显示出有前途的免疫性. 这种HPV疫苗配方在小鼠模型中成功诱导了中和抗体.

科学领域:

* 病毒学和免疫学
* 疫苗开发工作
* 分子生物学 * 分子生物学

背景情况:

* 人类乳头瘤病毒52型 (HPV52) 感染是一个重大的全球健康问题,特别是在东南亚的中低收入国家.
*预防性疫苗对于控制HPV感染和相关疾病至关重要.
*开发替代性和有效的HPV疫苗至关重要.

研究的目的:

* 评估由HPV 52 L1病毒样颗粒 (VLP) 制备的预防性候选疫苗的免疫性.
* 在小鼠模型中评估HPV 52 L1 VLP疫苗的疗效.
* 探索Hansenula polymorpha作为HPV疫苗生产表达系统的潜力.

主要方法:

* 在Hansenula polymorpha*中克隆和表达一个编码子优化的HPV 52 L1基因.
* 净化HPV 52 L1蛋白,并使用SDS-PAGE,西斑和TEM确认VLP形成.
* 用配方VLPs和辅助剂对BALB/c小鼠进行免疫接种,随后进行抗体标位分析 (ELISA) 和中和试验.

主要成果:

*成功生产和净化HPV 52 L1蛋白,该蛋白自组装成VLPs,回收率为51.76%.
* 疫苗配方诱导了高抗体标位,这表明小鼠模型中具有强大的幽默免疫反应.
*抗体在HEK293细胞中产生中和的HPV 52伪病毒感染.

结论:

* 汉森拉多态表达系统有效地产生HPV 52 L1 VLP,诱导显著的免疫反应.
* 开发的HPV 52 L1 VLP候选疫苗显示出中和HPV 52感染的潜力.
*这些发现支持进一步开发这种基于VLP的配方作为替代HPV疫苗.

关键词:

52型HPV型的人群.汉塞努拉多态植物 (Hansenula polymorpha) 是一种多态植物.免疫性免疫性免疫性这是一种L1蛋白质.中和测试试验中和测试试验

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