通过联合输送氧化和可纳来对抗同时发生的细菌和真菌感染
Rashmi Pandey1, Natalie Crutchfield1, Mark Richard Stephen Garren1
1School of Chemical, Materials, and Biomedical Engineering, College of Engineering, University of Georgia, Athens, Georgia 30602, United States.
ACS applied materials & interfaces
|April 14, 2025
概括
这项研究开发了一种结合氧化 (NO) 和可纳的新生物材料,以预防与设备相关的感染. 双作用涂层有效地减少了细菌和真菌的生长,为抗生素提供了一个有希望的替代品.
科学领域:
- 生物材料科学 生物材料科学
- 传染性疾病 传染性疾病
- 药物运输 药物运输 药物运输
背景情况:
- 与设备相关的感染给医疗保健带来了重大挑战,导致发病率,死亡率和经济负担.
- 细菌和真菌导致这些感染,形成生物膜,并对抗生素和抗真菌药物产生耐药性.
- 防止微生物粘附的生物材料为系统疗法提供了更安全的替代方案.
研究的目的:
- 开发和描述一种新的双输送系统,用于同时进行抗菌和抗真菌治疗.
- 在聚合物矩阵中将氧化 (NO) 和可纳结合起来,以预防与设备相关的感染.
主要方法:
- 通过将一氧化供体 (SNAP) 与聚碳酸尿 (TSPCU) 混合并用含可纳的聚乙烯尿 (TPU) 进行浸泡涂层,创建了一个复合生物材料.
- 使用水接触角 (WCA) 分析了表面特性.
- 在生理条件下,氧化和可纳释放动力学被测量了72小时. 对抗 Staphylococcus aureus , Escherichia coli 和 Candida albicans 的抗菌和抗真菌功效进行了测试. 使用小鼠纤维细胞来评估生物相容性.
主要成果:
- 复合材料表现出稳定的涂层和持续释放NO和可纳72小时.
- 观察到显著的细菌减少: >3-log对于*金黄色葡萄球菌*和>2-log对于*大肠杆菌*.
- 抗真菌活性显示 ~98%的粘附和 ~92%的浮游生物 *Candida albicans* 的减少. 该材料与小鼠纤维细胞细胞具有生物相容性.
结论:
- 开发的SNAP-fluconazole复合生物材料有效地同时对抗细菌和真菌感染.
- 这种双交付系统代表了对设备相关感染的有前途的预防策略.
- 这些发现支持进一步的临床应用的翻译研究.
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