膀和非膀细胞外小RNAs在与植物相互作用的细菌中直接抑制基因沉默
Antinéa Ravet1, Jérôme Zervudacki2,3, Meenu Singla-Rastogi1
1Institut de Biologie de l'ENS (IBENS), Ecole Normale Supérieure, Université PSL, CNRS, INSERM, F-75005, Paris, France.
Nature communications
|April 14, 2025
概括
植物可以使用细胞外小RNA (sRNA) 沉默细菌基因. 这一发现揭示了植物病原体相互作用和细菌基因调节的新机制.
科学领域:
- 植物病原体相互作用
- RNA生物学的RNA生物学
- 微生物基因组学 微生物基因组学
背景情况:
- 细胞外植物小RNAs (sRNAs) 和双链RNA (dsRNA) 前体在线状病原体中触发RNA干扰 (RNAi).
- 这些细胞外RNA物种在引导植物相互作用细菌中的基因沉默中的作用目前尚不清楚.
研究的目的:
- 调查细胞外植物sRNA是否可以诱导细菌中的基因沉默.
- 探索这种潜在的抗菌基因沉默 (AGS) 现象的机制和影响.
主要方法:
- 生产转基因阿拉比多普西斯植物表达sRNA,准Pseudomonas syringae毒性因子.
- 分析植物质中的细胞外sRNA种群,包括囊泡和非囊泡部分.
- 使用显微镜和测序检测细菌细胞内的sRNA.
- 研究酸 (SA) 在AGS中的作用.
主要成果:
- 表达特定sRNA的阿拉比多普西斯植物显示减少了Pseudomonas syringae的病变发生.
- 这种AGS现象依赖于Dicer-Like (DCL) 依赖的抗菌sRNA,而不是dsRNA前体.
- 确定了三种细胞外sRNA群体:与蛋白质相关的,细胞外囊泡 (EV) 封闭的和自由的细胞外sRNAs (efsRNAs).
- 在细菌细胞内发现了转基因和基因组衍生的efsRNA.
- 发现酸 (SA) 有助于促进AGS,内源性efsRNAs向由SA调节的细菌基因.
结论:
- 植物可以使用DCL依赖的sRNA来使细菌基因沉默,这是一个新的AGS机制.
- 细胞外自由sRNAs (efsRNAs) 是功能性的,可以进入细菌细胞.
- SA在促进AGS中发挥作用,在感染期间影响细菌基因表达.
- 这种AGS现象对理解植物微生物相互作用,微生物群落调节和细菌基因组进化有重大影响.
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