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染色体层次的基因组组合和笔Atrina pectinata的注释

Zhuanzhuan Li^1,2, Bo Liu³, Xi Chen^1,2

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, 266071, China.

Scientific data

|April 14, 2025

概括

为临灭绝的笔 (Atrina pectinata) 创建了一个高质量的基因组组. 这种资源有助于了解其繁殖生物学,并促进了对这种有价值的双动物的人工繁殖的努力.

科学领域:

海洋生物学海洋生物学
基因组学就是基因组学.
水产养殖是水产养殖的一种方式.

背景情况:

笔 (Atrina pectinata) 是一个在商业上重要的双物种,在东亚面临威胁.
人工繁殖A. pectinata对于水产养殖至关重要,但由于缺乏高质量的基因组而受到阻碍.
了解物种的生殖生物学是改进人工繁殖技术的关键.

研究的目的:

为了生成Atrina pectinata的高质量的染色体级基因组组合.
为A. pectinata研究提供一个基础的基因组资源.
促进该物种人工繁殖和保护方面的进步.

主要方法:

结合PacBio,Illumina和染色体形状捕获测序技术.
组装了一个951.01 Mb的染色体级基因组,一个52.64 Mb的N50支架.
标注了29326个编码蛋白质的基因,达到98.8%的BUSCO完整性.

主要成果:

成功将98.87%的基因组序列固定在17个染色体上.
鉴定并功能性注释了84.25%的预测蛋白质编码基因.
在基因组组装 (98.8%) 和基因预测 (97.7%) 两方面都获得了高完整性得分.

结论:

在Atrina pectinata的染色体级基因组组合是一个显著的进步.
这种基因组资源将加强对物种基因组学和生殖生物学的研究.
这项研究为改进人工繁殖策略和保护贝的保护工作奠定了基础.

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