直接RNA测序揭示了在对Campylobacter jejuni接种疫苗的反应中发生的转录后修饰
Yanan Zhao1, Yuanmei Wang1, Yanru Ren1
1Shandong Provincial Key laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, 271017, Shandong, China.
BMC genomics
|April 14, 2025
概括
这项研究揭示了Campylobacter jejuni感染如何改变的基因表达和RNA修饰. 关键发现突出了替代拼接,多元A尾和m6A/m5C修饰的变化,为免疫反应提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是一种主要的食源性病原体,影响家禽和公共健康.
- 转录后的修改对免疫和细胞功能至关重要.
- 在C. jejuni感染后,的表观遗传调节尚未得到充分理解.
研究的目的:
- 为了研究C. jejuni接种后肉肉的表皮转录学景观.
- 为了识别RNA转录的变化,基基修饰,替代拼接,和多种类型的尾巴长度.
- 探索表观遗传修饰在对C. jejuni.免疫反应中的作用.
主要方法:
- 在C. jejuni疫苗接种后,肉肉样本的直接RNA测序.
- 生物信息学和表达分析以表征RNA配置文件和基因修饰.
- 识别和分析新的转录,差异性的替代拼接,和多样性的尾部变化.
主要成果:
- 鉴定了超过4万个转录和23000个基因,其中包括超过1万个新的转录.
- 观察到替代拼接 (192个事件) 和多个A尾长 (426) 的显著变化.
- 差异表达的转录在免疫反应途径中得到了丰富,其中一些含有m5C和m6A修饰.
结论:
- 这项研究提供了C. jejuni感染后肉肉的转录后修饰的全面概况.
- ENSGALG00000012480和IFIH1被提议作为C. jejuni注射的潜在表观遗传标记.
- 这些发现增强了对宿主-病原体相互作用和免疫中的表转录酶调节的理解.
相关概念视频
03:21RNA-seq
9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.7K
02:24Ribosome Profiling
3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K
02:28Leaky Scanning
5.0K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.0K