相关实验视频

Updated: May 13, 2025

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

Published on: July 24, 2016

携带EV71病毒的微管穿过BBB通过内细胞通路诱导脑损伤

Xiaoyan Tian¹, Bingxin Liu¹, Linrun Li¹

¹Center for Public Health Research, Medical School, Nanjing University, 22 Hankou Rd, Nanjing, 210093, China.

Cell communication and signaling : CCS

|April 14, 2025

概括

微小水将Enterovirus 71传输穿过血脑屏障,恶化脑损伤. 微粒体内受损的线粒体促进了这一过程,为病毒性脑炎提供了一个新的治疗点.

科学领域:

神经病毒学神经病毒学
细胞生物学细胞生物学
病原体运输机制病原体运输机制

背景情况:

肠道病毒71 (EV71) 导致手足口病 (HFMD),并可能导致致命的神经并发症.
关于EV71神经侵袭和相关脑损伤的机制尚不清楚.

研究的目的:

为了研究微 (MVs) 在EV71穿越血脑屏障 (BBB) 的作用.
阐明MVs促进EV71神经侵入并诱导脑损伤的机制.

主要方法:

在体外BBB模型和体内成像以评估MV和EV71的运输.
qPCR用于量化血液和大脑组织中的病毒载量.
分析连接蛋白表达,MV吸收途径和线粒体损伤.
对NOX4衍生的ROS途径的研究.

主要成果:

携带EV71 (MVsEV71) 的MV在体外和体内有效地穿越了BBB.
MVsEV71感染导致大脑病毒载荷增加,脑出血和严重炎症.
MVs通过降低连接蛋白 (ZO-1,occludin) 来破坏BBB完整性.
MV内部化通过非克拉素依赖的途径发生,受损的线粒体被确定为关键载荷,促进BBB穿越和亡.

结论:

MVs作为EV71的载体,增强了整个BBB的神经侵入.
在MV中受损的线粒体在促进BBB透和加剧脑损伤方面发挥着至关重要的作用.
这些发现确定了EV71神经毒性的新机制,并建议MV和线粒体通路作为病毒性脑炎的潜在治疗点.

关键词:

血液-大脑屏障是什么?损坏的线粒体受损病毒EV71病毒细胞内存症是指细胞内存症.微小片是微小的片.

更多相关视频

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Published on: April 9, 2019

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

相关概念视频

Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

Intracellular Movement of Viruses and Bacteria

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...