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OsMbl1通过抑制其脂酶活性来对抗OsGdsl1-介导的米粉爆炸易感性

Linlin Song¹, Tao Yang², Yakubu Saddeeq Abubakar^1,3

¹State Key Laboratory of Agricultural and Forestry Biosecurity, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.

Plant, cell & environment

|April 15, 2025

概括

米蛋白OsGdsl1通过抑制ROS爆发来负面调节对Magnaporthe oryzae的免疫力. OsMbl1 抑制 OsGdsl1 脂酶活性,增强植物对真菌感染的防御能力.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
生物化学生物化学

背景情况:

植物莱克对抗病原体和昆虫的防御至关重要.
与米卡林相关的莱克丁OsMbl1对于模式触发免疫 (PTI) 至关重要,但其机制尚不清楚.

研究的目的:

为了阐明OsMbl1在大米免疫中的机制.
确定与OsMbl1相互作用的蛋白质及其在植物防御中的作用.

主要方法:

酵母两杂交测定以确定相互作用的蛋白质.
在体外和体内相互作用的研究.
基因表达分析 (RT-qPCR).
脂酶活动测定.
对基因删除突变体的表型分析.
反应性氧物种 (ROS) 爆裂试验.

主要成果:

一种类似GDSL的脂酶,OsGdsl1,被确定并确认与OsMbl1.1相互作用.
OsGdsl1表现出脂酶活性,并且局部存在脂质体和质体中.
OsGDSL1的表达受到Magnaporthe oryzae和植物激素的调节.
删除OsGDSL1突变体对M. oryzae表现出增强的耐药性,增加了ROS爆发,并上调了与病变发生相关的 (PR) 基因.
在M. oryzae感染期间,OsMbl1抑制了OsGdsl1脂酶活性.

结论:

OsGdsl1通过降低ROS爆发和PR基因表达的调节来负面调节大米对M. oryzae的免疫力.
OsMbl1 抑制 OsGdsl1 脂酶活性,从而增强大米对M. oryzae感染的天生的免疫力.

关键词:

马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.一个小小的东西.脂酶脂酶是一种植物免疫力植物免疫力米米饭米饭米饭米饭.

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