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两阶段适应性实验室进化增强了工程大肠杆菌的透耐受性,以改善酸盐生物合成

Yanzhe Shang¹, Zhengtong Zhu², Junru Sun²

¹MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, China.

Biotechnology journal

|April 15, 2025

概括

工程Escherichia coli ZZT215通过适应性实验室进化 (ALE) 改善离子耐受性,显示出增强的酸 (SA) 生产. 这种强壮的菌株实现了更高的产量和生产率,为工业化学合成提供了一个有前途的微生物平台.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
工业微生物学工业微生物学

背景情况:

酸 (SA) 是一种关键的平台化学物质,具有多种工业应用.
对于SA,大肠杆菌的发酵往往受到性中和剂的透应激的限制.
开发透耐受菌株对于高效的微生物SA生产至关重要.

研究的目的:

通过提高对透应激的耐受性来增强大肠杆菌的酸 (SA) 生产.
为了研究一种进化的菌株的代谢适应,以提高SA生产力.
为了证明适应性实验室进化 (ALE) 的有效性,用于设计强大的微生物细胞工厂.

主要方法:

采用两步的适应性实验室进化 (ALE) 策略来进化大肠杆菌菌株AFP111.1.
进行生物反应器发酵实验,以比较进化菌株 (ZZT215) 和母菌株 (AFP111) 之间的SA产量.
使用转录组分析来识别与改善的透耐受性相关的关键代谢变化.

主要成果:

进化菌株ZZT215与母菌株AFP111.1.相比,显著改善了Na+耐受性和SA生产力.
在5L生物反应器中,ZZT215在5L生物反应器中实现了87.02g/L的SA度峰值,生产率为1.01g/L·h.
转录组数据显示,ZZT215的TCA循环基因下调和ABC载体上调,表明适应了透应激.

结论:

多步骤ALE是一种有效的策略,用于开发透性强大的微生物菌株,以增强酸生产.
进化菌株ZZT215代表了SA.的工业规模微生物合成的有希望的候选人.
鉴定到的代谢适应提供了关于微生物细胞工厂在压力条件下进行高价值化学品生产的见解.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.适应性实验室进化的演变.葡萄糖酸是什么?葡萄糖酸是什么?转录组分析转录组分析两个阶段的发酵过程.

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