核变形和硬度依赖的引力生成决定了受限细胞的迁移
Zheng Wang1, Feng Xu2, Di Wu1
1Department of Mechanical Engineering, The University of Hong Kong, Central and Western District, Hong Kong SAR 999077, China.
ACS applied materials & interfaces
|April 15, 2025
概括
在狭窄的空间中,细胞迁移的速度取决于周围材料的刚度. 最佳的细胞运动发生在中间刚度,非常软和非常硬的环境阻碍迁移.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
背景情况:
- 细胞迁移对于胚胎发育和癌症转移至关重要.
- 了解物理约束,如限制尺寸和材料刚性如何影响细胞迁移是必不可少的.
- 目前对囚禁和硬度对细胞运动的综合影响的知识仍然有限.
研究的目的:
- 研究精确控制的微通道宽度和壁硬度对细胞迁移的影响.
- 阐明围墙刚度,核体积,肌酸蛋白表达和细胞迁移速度之间的关系.
- 开发一种模型,解释在不同的限制强度下观察到的细胞迁移行为.
主要方法:
- 使用无面膜光刻法和数字微镜装置 (DMD) 制造具有控制宽度和墙壁刚度的微通道.
- 在微通道内迁移的细胞中测量核体积和肌肉素表达水平.
- 在一系列壁度值中量化细胞迁移速度.
- 开发基于电机离合器的拉力赛车模型,以解释迁移动态.
主要成果:
- 限制壁硬度的增加降低了核体积,但没有影响肌肉素表达.
- 细胞迁移速度表现出双相趋势,在中间壁度 (约10kPa) 的最小速度.
- 一个电机离合器模型解释了这种两相行为,将核变形,细胞壁摩擦和肌驱动的爬行力联系起来.
结论:
- 在封闭的环境中,细胞迁移受到封闭硬度和细胞力学之间的相互作用的显著调节.
- 双相迁移反应表明,对于有效的细胞运动来说,最优的刚性是理想的,偏差导致速度降低.
- 这些发现为控制细胞运动和设计用于组织工程和治疗应用的先进生物材料提供了洞察力.
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