通过使用MALDI-TOF MS标记的1-pyrenecarboxaldehyde标记的氨酸和同氨酸的同时定量检测.
Liming Guo1,2, Hao Wang3, Liang Song2
1State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
Journal of the American Society for Mass Spectrometry
|April 15, 2025
概括
这项研究引入了一种使用1-pyrenecarboxaldehyde的新方法,用于通过MALDI-TOF MS.量化囊素 (Cys) 和同类囊素 (Hcy). 该方法在没有内部标准的生物样本中实现了高灵敏度和精度.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 半氨酸 (Cys) 和同氨酸 (Hcy) 是重要的生物醇,参与许多生理过程.
- 准确量化Cys和Hcy对于理解它们的生物学作用至关重要.
研究的目的:
- 开发一种灵敏而准确的方法,用于同时量化Cys和Hcy.
- 使用1-pyrenecarboxaldehyde (1-py) 衍生来提高通过MALDI-TOF MS.的检测.
主要方法:
- 用1-pyrenecarboxaldehyde (1-py) 来衍生Cys和Hcy.
- 使用矩阵辅助激光脱落和离子化飞行时间质谱法 (MALDI-TOF MS) 进行定量分析.
- 方法验证,包括检测极限,线性范围和应用于细胞溶解物.
主要成果:
- 对Cys和Hcy.的低检测极限达到250amol/L.
- 既定的线性定量范围从5到2500μM不包括内部标准.
- 在HepG2细胞溶解酸中成功量化Cys和Hcy.
结论:
- 与MALDI-TOF MS相结合的1-py衍生法为生物醇量化提供了快速,灵敏和准确的方法.
- 这种方法为涉及Cys和Hcy的生物和临床研究提供了有价值的工具.
更多相关视频
07:16Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
1.6K
12:11Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
6.7K
相关概念视频
Peptide Identification Using Tandem Mass Spectrometry
6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
Mass Spectrometry: Complex Analysis
653
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
653
