相关实验视频

Updated: May 13, 2025

Experimental Infection of Mice with the Parasitic Nematode Strongyloides ratti ratti Infection

Experimental Infection of Mice with the Parasitic Nematode Strongyloides ratti ratti Infection

Published on: January 17, 2025

免疫蛋白-3通过结合宿主血细胞和幼虫表面来调解寄生虫幼虫的封装

Anmin Zhao^1,2, Shuyan Li^1,2, Ziyao Wang^1,2

¹Shenzhen Campus of Sun Yat-sen University, Shenzhen, P. R. China.

Pest management science

|April 15, 2025

概括

在Ostrinia furnacalis中的免疫蛋白-3 (OfIML-3) 对于封装寄生虫幼虫至关重要. 击败OfIML-3显著降低了封装,揭示了其在宿主免疫防御对寄生虫的作用.

科学领域:

昆虫免疫学昆虫免疫学
跨物种的相互作用.
主体防御的分子机制

背景情况:

寄生虫是昆虫,它们在宿主体内或上面产卵,最终杀死宿主.
了解宿主如何抵抗寄生虫是昆虫共同进化的关键.
封装反应是对寄生虫的主要宿主防御机制.

研究的目的:

研究immulectin-3 (OfIML-3) 在Ostrinia furnacalis寄生虫幼虫封装中的作用.
阐明OfIML-3促进宿主免疫反应的分子机制.

主要方法:

使用RNA干扰 (RNAi) 来降低O. furnacalis.中的OfIML-3表达.
为了评估OfIML-3完全缺少OfIML-3,生成了同卵性突变菌株 (OfIML-3-/-).
在移植到原始和OfIML-3缺乏宿主后,量化了幼虫封装率.
研究了OfIML-3在血细胞和寄生虫幼虫上的定位.

主要成果:

OfIML-3主要表达在脂肪体中,并分泌到血中,在寄生虫攻击时诱导表达.
淘汰OfIML-3减少了寄生虫幼虫封装从88%降至37%,而同卵性突变只显示了7%的封装.
在宿主血细胞和寄生虫幼虫表面检测到OfIML-3,这表明它直接参与识别和结合过程.

结论:

OfIML-3作为一个关键的识别因子,促进了O. furnacalis.寄生虫幼虫的封装.
这项研究提供了关于昆虫封装机制和寄生虫宿主共同进化的见解.
OfIML-3的功能突出了昆虫免疫防御对寄生虫入侵者的关键策略.

关键词:

这就是CRISPR/Cas9的作用.在C型乳清素中,这种虫是Ostrinia furnacalis.封装的封装方式血球细胞是血液细胞的组成部分.

更多相关视频

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

Published on: April 6, 2015

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Published on: May 7, 2012

相关概念视频

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Transcytosis of IgG

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...

Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

Selectins

Selectins

Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...