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来自Codonopsis pilosula的超声波辅助多糖体的结构特征和生物活性分析:对抗氧化和低血糖潜力的洞察

Anxiang Wang¹, Hong Zhang¹, Mingjun Yang¹

¹Lanzhou University of Technology, School of Life Science and Engineering, Pengjiaping Road 36, Qilihe District, Lanzhou City, Gansu Province, 730050, Lanzhou City, CHINA.

Chemistry & biodiversity

|April 15, 2025

概括

超声波辅助提取产生了六种Codonopsis pilosula多糖体 (UA-Cpps). UA-Cpps中的1→4糖键有助于抗氧化活性,抑制潜在健康益处的关键酶.

科学领域:

药理学药理学是指药理学的学科.
生物化学生物化学
食品科学食品科学食品科学

背景情况:

科多诺普西斯 (Codonopsis pilosula) 是一种公认的药用和食用植物.
在C. pilosula中,多糖体结构与活性之间的关系仍未得到充分研究.

研究的目的:

从C. pilosula.中提取和描述多糖类.
研究这些多糖的物理化学性质,结构和生物活性.

主要方法:

超声波辅助提取 (UAE) 的原始多糖.
DEAE-纤维素和S-100染色学用于净化.
高分辨率光谱,扫描电子显微镜,甲基化分析和分子对接用于表征和活动评估.

主要成果:

六种不同的多糖化合物 (UA-Cpps) 被分离和特征.
形态分析显示了UA-Cpps1和UA-Cpps6.的密集,由粒子组成的表面.
甲基化分析表明了主要的1→4和1→6糖化链接.
1→4链接与增强的抗氧化特性相关.
UA-Cpps3和UA-Cpps5表现出显著的抗氧化活性和α-氨酶和α-葡萄糖酶的抑制.
分子对接证实了UA-CPPs和目标酶之间的稳定相互作用.

结论:

这项研究提供了对C. pilosula多糖 (UA-Cpps) 的全面分析.
这些发现突出了1→4甘氨酸键在抗氧化剂有效性中的作用.
这些多糖体显示出促进健康和管理代谢健康问题的潜力.

关键词:

降低血糖的活动.C. pilosula 是一种多糖化合物.分子对接是分子对接.氧化应激抑制活性氧化应激抑制活性结构的表征结构的表征.

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