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Updated: May 13, 2025

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使用毛细管区电泳,对抗体-寡核酸合物具有不同的固态度的无标签量化量化

Mo Zhou^1,2, Xiaoxia Zhang³, Qinglin Xia^1,2

¹CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

|April 15, 2025

概括

一种新的毛细电泳法量化了抗体-寡核酸合物 (AOC) 变异. 这种技术精确地分析了结合效率和寡核酸与抗体比率 (OAR) 的异质性.

科学领域:

生物结合化学生物结合化学
分析化学分析化学
生物物理学的生物物理.

背景情况:

抗体-寡核酸合物 (AOC) 在诊断和治疗中至关重要.
描述AOC异质性对于质量控制和理解生物功能至关重要.
现有的方法往往缺乏详细分析所需的分辨率或定量准确性.

研究的目的:

开发一个无标签的毛细管区电泳 (CZE) 平台,用于AOC的高分辨率分析.
量化评估AOC的异质性,包括结合效率和寡核酸与抗体比率 (OAR).
基于其产生的异质性来比较AOC合成策略.

主要方法:

实施一个无标签的毛细管区域电泳 (CZE) 系统.
开发用于分解自由抗体,自由寡核酸和各种AOC亚群的方法.
使用CZE数据对效率和OAR进行定量分析.

主要成果:

该CZE平台实现了单链寡核酸 (21nt) 分辨率.
成功地解决了AOC,自由抗体和自由寡核酸的不同亚群.
在不同合成方法中提供精确的对联效率和OAR异质性的定量评估.

结论:

没有标签的CZE是用高分辨率来描述AOC异质性的强大工具.
开发的平台可以准确评估合效率和OAR,这对于AOC开发至关重要.
这种方法有助于优化AOC合成策略,以提高产品的一致性.

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