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针对CD155的双特异性抗体介导T细胞免疫疗法对抗人类妇科恶性瘤

Li Ma¹, Juan Ma², Dingqing Feng³

¹Department of Gynecology and Obstetrics, China-Japan Friendship Hospital, Capital Medical University, Beijing, 100029, China. mali@zryhyy.com.cn.

Investigational new drugs

|April 15, 2025

概括

这项研究表明,将双特异抗体 (Bi-Ab) 针对妇科瘤的CD155有效地激活T细胞以杀死癌细胞. CD155Bi-T细胞在宫,子宫和卵巢癌中显著增强瘤杀伤活性.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

由于其细胞毒性能力,T细胞对于癌症治疗至关重要.
双特异性抗体 (Bi-Ab) 是新兴的免疫疗法,可以招募和激活T细胞以消除瘤.
CD155作为妇科恶性瘤治疗点的潜力需要研究.

研究的目的:

确定CD155是否是人类妇科癌症T细胞介导免疫治疗的可行的标.
为了评估与CD155向双特异性抗体 (CD155Bi-Ab) 设计的T细胞对妇科瘤细胞的疗效.

主要方法:

对宫,子宫和卵巢癌细胞进行了CD155表达的评估.
用CD155Bi-Ab武装T细胞来创建CD155Bi-T细胞.
CD155Bi-T细胞的细胞毒性活动与使用 luciferase assays 的对照T细胞进行了比较.
分析了T细胞激活标志物的表达和细胞因子的产生.

主要成果:

CD155在常见的妇科瘤细胞上得到表达.
与对照T细胞相比,CD155Bi-T细胞对CD155阳性瘤细胞显著增强了细胞毒性 (E:T比率为5:1的2.67倍).
增强的活性与增加的CD69和4-1BB表达,增加的IL-2,IFN-γ和TNF-α产量以及更高水平的穿孔素和B粒酶相关.

结论:

CD155代表了对妇科瘤的有前途的治疗标.
CD155Bi-T细胞表现出强大的抗瘤活性,并具有治疗CD155阳性妇科恶性瘤的显著潜力.

关键词:

双特异性抗体双特异性抗体双特异性抗体 CD155 是一个CD155 是一个CD155 细胞毒性细胞毒性妇科恶性瘤妇科恶性瘤

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