相关实验视频

Updated: May 23, 2025

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

系统性硬化皮肤病原性非迁移性纤维细胞群的表征

Kristina En Clark^1,2, Shiwen Xu¹, Moustafa Attar²

¹Centre for Rheumatology, Division of Medicine, University College London, London, United Kingdom.

|April 15, 2025

概括

系统性硬化症 (SSc) 涉及两种纤维细胞种群:迁移性和寄居性. 迁移细胞通过TGF-β信号驱动早期纤维化,而定居细胞则在以后作出贡献,表达化基因,提供新的治疗点.

科学领域:

免疫学免疫学免疫学
皮肤病学皮肤病学
细胞生物学细胞生物学

背景情况:

纤维细胞是系统性硬化症 (SSc) 发病的关键.
传统的纤维细胞培养可能不能完全代表SSc生物学或治疗反应.

研究的目的:

识别和描述SSc皮肤中不同的纤维细胞群.
为了比较SSc.中的迁移性纤维细胞和寄居性纤维细胞之间的功能和分子差异.
调查新发现的纤维细胞种群的潜在治疗向性在SSc.

主要方法:

从SSc皮肤活检中分离移徙和非移徙 (居民) 纤维细胞群.
功能性检测,大批和单细胞RNA测序 (RNA-seq).
多通道免疫光用于局部定位.

主要成果:

在SSc.中发现了两种不同的纤维细胞种群,一种是迁徙的,一种是寄居的.
迁移性纤维细胞在早期扩散性皮肤SSc (dcSSc) 中显示出高TGF-β信号.
居住性纤维细胞表现出增加的化学激素表达,并且更多地涉及到晚期纤维化.

结论:

迁移性纤维细胞在早期的SSc中至关重要,而定居性纤维细胞可能发挥调节作用,并导致晚期纤维化.
在SSc中以前未被识别的致病性纤维细胞群提供了潜在的治疗点.
不同的纤维细胞对TGF-β1和CCL2的反应突出显示了SSc病变发生过程中的不同作用.

关键词:

自身免疫性疾病是一种自身免疫性疾病.这是一种自身免疫力.纤维化纤维化纤维化免疫学免疫学免疫学炎症炎症是一种炎症.

更多相关视频

Murine Dermal Fibroblast Isolation by FACS

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...