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Updated: May 13, 2025

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关于在体外分离CIDEC蛋白的N端的N相分离的协议

Yiming Lu1, Zanzan Zhu1, Jianqin Wang2

  • 1Shanghai Key Laboratory of Metabolic Remodeling and Health, State Key Laboratory of Genetics and Development of Complex Phenotypes, Institute of Metabolism and Integrative Biology, School of Life Sciences, Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai 200438, China.

STAR protocols
|April 15, 2025
PubMed
概括

研究人员开发了一种用于体外分离CIDEC-N蛋白的分相协议. 这种方法有助于研究诱导细胞死亡的DFF45样效应因子C.

关键词:
生物物理学的生物物理.代谢 代谢 代谢 代谢蛋白质表达和净化过程

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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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