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通过CAGE和纳米孔测序绘制病毒病原体的时间转录组签名

Dóra Tombácz¹, Balázs Kakuk¹, Gábor Torma¹

¹Department of Medical Biology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary.

|April 15, 2025

概括

马性疹病毒1 (EHV-1) 基因表达随着时间的推移而被描述,揭示了新的转录和完善其基因组注释. 这种对EHV-1复杂的调控网络的先进理解增强了对病毒复制的知识.

科学领域:

病毒学病毒学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

马类阿尔法疹病毒1 (EHV-1) 在马类产业中造成重大经济损失.
EHV-1是一种兽医病原体,属于水病毒属.

研究的目的:

为了执行EHV-1的时间尺度基因表达概况.
为了实现EHV-1基因组的转录性重新注释.

主要方法:

使用 CAGE 测序 (Illumina) 进行转录开始站点.
采用长读直接cDNA测序 (牛津纳米孔) 进行全长的转录.
集成的CAGE和直接的cDNA测序数据与蛋白质合成抑制和原生RNA测序.

主要成果:

在整个复制周期中表征了EHV-1转录动力学.
识别和验证了许多新的转录,完善了EHV-1转录组注释.
精确地绘制了EHV-1转录组图,揭示了同型多样性和促进体区域.

结论:

EHV-1 呈现出一个复杂的调节网络,具有转移的转录异型和重叠.
每个基因的多个转录异型允许EHV-1在感染期间适应和增强基因组效率.
这种适应性使得基因表达和对宿主环境的反应能够更有效地调节.

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