李斯特菌需要光转移酶系统来促进细胞内生长和毒性
Matthew J Freeman1, Noah J Eral1, John-Demian Sauer1
1Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
PLoS pathogens
|April 15, 2025
概括
李斯特菌使用光转移酶系统 (PTS) 进行毒性,而不仅仅是糖醇和酸. 破坏PTS会损害宿主中的细菌生长和毒性.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 代谢工程是代谢工程.
背景情况:
- 细菌病原体在营养有限的环境中具有专门的新陈代谢,用于宿主毒性.
- 一种细胞系致病原体Listeria monocytogenes被认为使用双重新陈代谢,利用甘油和酸用于不同的细胞需求.
- 这种双重代谢在L. monocytogenes毒性中的作用仍然不完全理解.
研究的目的:
- 为了研究糖醇和酸代谢在L. monocytogenes毒性中的作用.
- 确定L. monocytogenes在细胞内生长过程中使用的替代碳来源.
- 确定转移酶系统 (PTS) 对L. monocytogenes细胞内生长和毒性的贡献.
主要方法:
- 构建和体内/体内对缺乏关键代谢基因 (ΔglpD/ΔgolD, ΔuhpT, ΔptsI, ΔptsH) 的L. monocytogenes突变体的体内/体内表征.
- 使用BioLog进行表型微阵列分析,以评估碳源利用率.
- 在宿主模型中评估细菌细胞内生长和毒性减弱.
- 使用构成性活性毒性调节剂 (PrfA*) 的补充研究.
主要成果:
- 不能代谢甘或酸的L. monocytogenes突变体在体内显著减弱,表明依赖替代碳来源.
- 代谢查显示,L. monocytogenes利用转移酶系统 (PTS) 介导的碳来源,挑战了以前的模型.
- 基本PTS组件 (PtsI和PtsH) 的突变严重损害了细胞内生长和毒性,与糖醇/酸突变相结合时具有添加效应.
结论:
- L. monocytogenes 采用 PTS 介导的碳源利用用于细胞内生长和毒性,除了糖醇和酸.
- PTS组成部分 (PtsI和PtsH) 是细胞质细菌生长的关键毒性因素.
- 代谢灵活性,特别是通过PTS,对于L. monocytogenes在宿主细胞质中的病原体是必不可少的.
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