使用tigecycline向癌细胞线粒体改善了结直肠癌细胞系的放射治疗反应

使用tigecycline向癌细胞线粒体改善了结直肠癌细胞系的放射治疗反应

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Sepideh Hassanpour Khodaei¹, Shahnaz Sabetkam², Zeinab Mazloumi³

¹Department of Dentistry, Eastern Mediterranean University (EMU), Mersin 10, Famagusta, North Cyprus, Turkey.

Mutation research

|April 15, 2025

概括

作为线粒体抑制剂的tigecycline增强了结直肠癌细胞对放射治疗的敏感性. 组合疗法增加了亡和caspase-3活性,提供了一种改善结直肠癌治疗结果的新方法.

科学领域:

在瘤学瘤学.
癌症生物学癌症生物学
辐射疗法辐射疗法

背景情况:

结肠直肠癌 (CRC) 是全球癌症相关死亡的主要原因.
在CRC中治疗耐药性通常归因于癌症干细胞.
由于治疗选择有限和预后不佳,新的治疗策略至关重要.

研究的目的:

为了研究Tigecycline,一种线粒体抑制剂,结合放射治疗对结直肠癌细胞亡的作用.
为了评估tigecycline的潜力,使癌细胞对辐射疗法的敏感.

主要方法:

人类结直肠癌细胞 (HCT-116) 用tigecycline进行治疗,并使用MTT测定来评估生存能力.
细胞使用线性加速器暴露于400cGy辐射.
使用AnnexinV/PI染色,流细胞计和基因表达分析来评估亡,caspase-3活性和亡基因的表达.

主要成果:

在48小时后,tigecycline的IC50值为93μM.
在IC50值的线粒体抑制使结直肠癌细胞对放射治疗敏感.
与单一疗法相比,组合疗法显著增加了亡细胞和caspase-3活性.
支持apoptotic的基因被调高,而反apoptotic的基因被调低.

结论:

用tigecycline针对线粒体可以增强结直肠癌细胞对治疗的敏感性.
这种方法可能提供一种临床相关的策略,以改善CRC治疗.
这些发现为开发新的结直肠癌疗法提供了新的见解.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.结肠直肠癌是一种癌症.辐射疗法辐射疗法提格环素是一种提格环素.

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