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使用体外,体内和计算模型探索Vigna sesquipedalis的抗糖尿病潜力
Hammad Ahmad1, Ashraf Ullah Khan2, Waqas Alam1
1Department of Pharmacy, Abdul Wali Khan University, Mardan, 23200, Mardan, Pakistan.
Journal of computer-aided molecular design
|April 15, 2025
概括
维格纳·塞斯基佩达利斯 (Vigna sesquipedalis) 提取物通过改善小鼠的体重和血液标志物,显示出显著的抗糖尿病潜力. 分子对接和模拟表明,提取物中的化合物与关键的糖尿病相关酶和蛋白质产生良好的相互作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 自然产品化学 自然产品化学
- 生物化学 生物化学
背景情况:
- 维格纳·塞斯基佩达利斯 (Vigna sesquipedalis) 传统上用于糖尿病治疗,但没有科学验证.
- 民族医学用途需要对潜在的治疗益处进行科学研究.
研究的目的:
- 为了评估Vigna sesquipedalis的抗糖尿病和抗氧化潜力.
- 通过体外,体内和计算研究来研究其抗糖尿病作用背后的分子机制.
主要方法:
- 通过DPPH和ABTS测定评估的抗氧化活性.
- 通过α-葡萄糖酶和α-氨基酶抑制评估的体外抗糖尿病潜力.
- 在体内研究中使用了一种链毒素诱导的糖尿病小鼠模型,包括毒性测试.
- 计算方法包括虚拟选,分子动力学 (MD) 模拟和结合自由能计算.
主要成果:
- 乙酸乙烯分量显示出最高的抗氧化活性,而甲醇原料提取物表现出最强的体外抗糖尿病作用.
- 原始提取物高达2000mg/kg体重是无毒的,并且在糖尿病小鼠中显著改善了体重.
- 在体内研究显示出显著的抗糖尿病作用,由肝糖原,血清胰岛素,糖化血红蛋白和组织病理学的改善所支持.
- 虚拟查发现,氨,多醇和psoralidin与氨基酶,GLP1和葡萄糖酶分别具有很高的结合亲和力,MD模拟和MM-PBSA/GBSA分析证实了稳定的相互作用.
结论:
- 维格纳·塞斯基佩达利斯 (Vigna sesquipedalis) 具有显著的抗氧化和抗糖尿病特性.
- 这项研究提供了科学证据,支持Vigna sesquipedalis在糖尿病管理中的传统用途.
- 维格纳·塞斯基佩达利斯 (Vigna sesquipedalis) 中的化合物显示出作为治疗胰岛素依赖糖尿病的药物剂的前景.
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