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Updated: May 13, 2025

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通过快速泛膜蛋白标记揭示细胞通信

Hirushi Gunasekara1, Yu-Shiuan Cheng1, Vanessa Perez-Silos2

  • 1Department of Chemistry, College of Liberal Arts and Sciences, University of Illinois Chicago, Chicago, IL, 60607, USA.

Nature communications
|April 15, 2025
PubMed
概括

这项研究引入了一种用于活细胞表面蛋白质标记的新型氨酸交联方法,使高密度多重成像成为可能. 这项技术可视化了各种细胞类型和体内活体中的动态细胞相互作用和蛋白质贩运.

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Protein Dynamics in Living Cells01:19

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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