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大口 (Micropterus salmoides) 的基因组组合草案

Tao Zhu^1,2, Hongmei Song¹, Jinxing Du¹

¹Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.

BMC genomic data

|April 15, 2025

概括

研究人员对大鱼 (Micropterus salmoides) 的基因组进行了测序,生成了高质量的草稿组装. 这项基础工作支持这一关键水产养殖物种的遗传和育种进步.

科学领域:

水产养殖基因组学水产养殖基因组学
鱼类遗传学鱼类遗传学
基因组组装的基因组组装

背景情况:

大鱼 (Micropterus salmoides) 是一个重要的水产养殖物种,产量不断增加.
高质量的基因组组合对于推进水产养殖中的遗传和育种研究至关重要.

研究的目的:

为了生成一个高质量的基因组组装,男性大鱼 (Micropterus salmoides).
为大鱼的遗传和育种研究提供一个基础资源.

主要方法:

基因组测序使用PacBio Sequel,高通量染色体构造捕获 (Hi-C) 和Illumina配对末端测序.
通过异形测序 (Iso-Seq) 进行全长的转录测序.
生态信息组合和对生成的基因组数据的注释.

主要成果:

一个877.7 Mb的基因组组合草案,N50为37.2 Mb,包括23个染色体和202个支架.
鉴定了23952个编码基因和注释重复序列 (32.8%的基因组).
产生大量的测序数据:164.5Gb (PacBio),113.4Gb (Hi-C),54.7Gb (Illumina) 和22.8Gb (Iso-Seq) 的数据.

结论:

成功获得了雄性大嘴的基因组组合草案.
这种基因组资源对于未来在大嘴鱼水产养殖中的遗传和育种应用至关重要.

关键词:

水产养殖是水产养殖的一种方式.基因组注释基因组注释基因组组装组的基因组组装组大嘴巴低音是什么意思

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Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Lampbrush Chromosomes

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In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
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