相关实验视频

Updated: May 13, 2025

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

转录组分析显示,长非编码RNA参与了对果中Botrytis cinerea的反应中的关键代谢途径

Yijia Ma¹, Tianjing Zeng¹, Zhexin Li²

¹Chongqing Key Laboratory for Germplasm Innovation of Special Aromatic Spice Plants, Collaborative Innovation Center of Special Plant Industry in Chongqing, College of Smart Agriculture, Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan, 402160, China.

BMC plant biology

|April 15, 2025

概括

长非编码RNAs (lncRNAs) 在果对Botrytis cinerea的防御中起着至关重要的作用. 这项研究确定了调节植物激素和MAPK信号通路的lncRNAs,增强了水果对真菌疾病的抵抗力.

科学领域:

植物科学植物科学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

了解果对 Botrytis cinerea 的耐药性,是开发耐药品种的关键.
长非编码RNAs (lncRNAs) 在果对抗疾病的防御机制中的作用尚不清楚.

研究的目的:

为了识别由Botrytis cinerea感染引起的"Hongyang"桃中的lncRNAs.
阐明疾病反应期间由这些 lncRNAs 调节的分子通路.

主要方法:

高通量测序用于识别 lncRNAs.
差异表达分析以找到由B. cinerea.诱导的lncRNAs.
基因本体学 (GO) 和KEGG途径分析以确定目标基因的功能.
共同表达网络分析以了解监管角色.

主要成果:

确定了126个差异表达的lncRNAs (DELs).
DELs的目标基因参与植物病原体相互作用,碳水化合物代谢,MAPK信号传递和植物激素信号转导.
在MAPK通路的同时,lncRNAs调节了auxin,乙烯,ABA,JA和SA信号通路.
这种调节会影响防御代谢物的合成,增强果的耐药性.

结论:

lncRNAs是基维果生物应激反应的重要调节者.
这些发现提供了对lncRNA介导的防御机制的洞察力,以对抗B. cinerea.
这项研究可以为提高果抗病能力的育种策略提供信息.

关键词:

这种植物是Botrytis cinerea.果 (Kiwi) 是一种果.在 LncRNAs 中.电阻电阻电阻电阻电阻电阻

更多相关视频

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Published on: July 2, 2018

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...