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循环瘤细胞的检测是通过流细胞计在黑色素瘤患者中实现的

Ludivine Fourdrain¹, Théo Brochet², Valentin Clichet³

¹Service d'Hématologie Biologique, CHU Amiens-Picardie, Amiens, France.

Cytometry. Part B, Clinical cytometry

|April 16, 2025

概括

循环瘤细胞 (CTC) 可以在所有黑色素瘤患者中检测到,即使在早期阶段. 它们的数量在转移性黑色素瘤中显著增加,突出显示它们作为预后标记物的潜力.

科学领域:

在瘤学瘤学.
皮肤病学皮肤病学
细胞生物学细胞生物学

背景情况:

黑色素瘤的发病率正在上升,需要新的预后因素.
循环瘤细胞 (CTC) 通过液体活检提供了瘤材料的非侵入性来源.
目前的CTC检测主要依赖于分子生物学技术.

研究的目的:

用流式细胞计量来研究黑色素瘤患者中CTC的检测.
建立流细胞计作为在黑色素瘤中检测CTC的可行方法.
探索CTC和黑色素瘤预后因素之间的相关性.

主要方法:

预期的,纵向研究设计.
流细胞测量技术用于CTC检测.
对黑色素瘤细胞的高灵敏度和特异性标记策略.

主要成果:

所有35名患者在纳入时都有可检测的CTC,无论疾病的阶段如何.
在转移与非转移阶段观察到CTC计数显著增加.
在CTC计数和Breslow厚度或之间没有发现显著的关联.

结论:

流细胞计是检测黑色素瘤患者中CTC的一种敏感方法.
在黑色素瘤的所有阶段都存在CTC,在转移性疾病中数量增加.
需要对更大的队列和更长的随访进行进一步的研究,以确认CTCs的预后意义.

关键词:

循环中的瘤细胞.检测检测检测检测检测流动细胞计量是流动细胞计量的方法.黑色素瘤是一种黑色素瘤.

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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...