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含氨酸的自组装β片对巨细胞极化和炎症反应的影响

Jacek K Wychowaniec¹, Ezgi Irem Bektas¹, Marcia Muerner^1,2

¹AO Research Institute Davos, Clavadelerstrasse 8, Davos 7270, Switzerland.

ACS applied materials & interfaces

|April 16, 2025

概括

自组合 (SAP) 调节免疫反应. 像EF8这样的特定序列促进了抗炎M2巨的两极分化,而YEF8诱导了亲炎M1巨,为免疫调节纳米生物材料提供了潜力.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
免疫学免疫学免疫学

背景情况:

自组装 (SAP) 是具有可调节结构的多功能纳米生物材料.
控制SAPs的纳米结构和化学特性是操纵细胞信号的关键.
了解SAP对先天免疫系统炎症信号的影响至关重要.

研究的目的:

设计和合成五个具有不同氨酸位置的β-sheet SAP.
研究这些SAP的化学和形态特征如何影响炎症信号.
探索SAPs作为免疫调节剂的潜力.

主要方法:

合成了五种β-sheet SAPs:EF8,YEF8,EF8Y,YEF8Y和EYF8,它们的定位不同.
描述了SAP自组装成纳米纤维及其捆绑性质.
使用THP-1细胞和初级人类PBMCs评估了炎症反应和巨分化 (M1/M2).

主要成果:

所有设计的SAP都形成了纳米纤维;YEF8和EF8显示捆绑.
可溶性EF8纳米纤维诱导了抗炎M2极化.
可溶性YEF8纳米纤维诱导了亲炎性M1极化.
EF8Y,YEF8Y和EYF8没有引起显著的炎症反应.
结果在THP-1细胞和人类PBMC以及水凝培养物中一致.

结论:

序列,特别是氨酸的位置,决定了SAP的自我组装和免疫调节活性.
EF8 和 YEF8 SAP 可以分别用作抗炎 (M2) 或促炎 (M1) 剂.
这些发现支持SAPs作为可调节的免疫调节纳米生物材料的开发,用于炎症疾病的治疗应用.

关键词:

免疫调节免疫调节免疫调节这是一种炎症炎症炎症炎症.巨细胞的两极分化纳米结构类的.用氨酸修饰的自组合.

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