相关实验视频

Updated: May 13, 2025

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
08:33

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Published on: March 11, 2021

1.9K

该BK通道-NS1619激素复合体揭示了分子洞察力关于全激活门的分子洞察力

Naileth Gonzalez-Sanabria, Gustavo F Contreras, Maximiliano Rojas

    bioRxiv : the preprint server for biology
    |April 16, 2025
    PubMed
    概括

    研究人员发现NS1619是如何通过结合特定的口袋来激活BK通道的,从而稳定开放的孔隙. 这一发现为开发用于治疗BK通道功能障碍的新疗法提供了有希望的目标.

    更多相关视频

    Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes10:19

    Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

    Published on: January 10, 2011

    20.9K
    Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells15:28

    Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

    Published on: October 1, 2010

    17.4K

    相关概念视频

    Allosteric Regulation01:08

    Allosteric Regulation

    Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
    57.2K
    Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

    Ligand-Gated Ion Channel Receptor: Gating Mechanism

    Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
    2.0K
    Cooperative Allosteric Transitions01:58

    Cooperative Allosteric Transitions

    2.3K
    Ligand-gated Ion Channels01:19

    Ligand-gated Ion Channels

    Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
    Three Subfamilies of Ligand-gated Ion Channels
    Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
    12.0K
    Non-gated Ion Channels01:24

    Non-gated Ion Channels

    Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
    Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
    6.6K
    Ligand Binding and Linkage00:49

    Ligand Binding and Linkage

    Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
    4.7K